State Key Joint Laboratory of Environmental Simulation and Pollution Control, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China; Department of Water Pollution Control Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China.
Nanchang University, Nanchang 100123, PR China.
Bioresour Technol. 2016 Sep;216:1049-57. doi: 10.1016/j.biortech.2016.06.012. Epub 2016 Jun 4.
Swine manure has been considered as the reservoir of antibiotic resistance genes (ARGs). Composting is one of the most suitable technologies for treating livestock manures, and red mud was proved to have a positive effect on nitrogen conservation during composting. This study investigated the abundance of eight tetracycline and three copper resistance genes, the bacterial community during the full scale swine manure composting with or without addition of red mud. The results showed that ARGs in swine manure could be effectively removed through composting (reduced by 2.4log copies/g TS), especially during the thermophilic phase (reduced by 1.5log copies/g TS), which the main contributor might be temperature. Additionally, evolution of bacterial community could also have a great influence on ARGs. Although addition of red mud could enhance nitrogen conservation, it obviously hindered removal of ARGs (reduced by 1.7log copies/g TS) and affected shaping of bacterial community during composting.
猪粪一直被认为是抗生素抗性基因（ARGs）的储存库。堆肥是处理牲畜粪便最适宜的技术之一，而赤泥已被证明在堆肥过程中对氮的保存有积极作用。本研究调查了在添加或不添加赤泥的情况下，规模化猪粪堆肥过程中 8 种四环素和 3 种铜抗性基因的丰度以及细菌群落的变化。结果表明，通过堆肥（TS 减少 2.4log 拷贝/g）可以有效去除猪粪中的 ARGs，特别是在高温阶段（TS 减少 1.5log 拷贝/g），主要贡献因素可能是温度。此外，细菌群落的演替也会对 ARGs 产生很大的影响。虽然添加赤泥可以促进氮的保存，但明显会阻碍 ARGs 的去除（TS 减少 1.7log 拷贝/g），并影响堆肥过程中细菌群落的形成。
