Biomaterials Department, Faculty of Oral and Dental Medicine, Future University in Egypt, New Cairo, 11865, Egypt.
Oral Pathology Department, Faculty of Oral and Dental Medicine, Future University in Egypt, New Cairo, 11865, Egypt.
Sci Rep. 2024 Aug 27;14(1):19855. doi: 10.1038/s41598-024-69836-z.
Soft denture liners have limitations like short lifespan and increased microbial buildup. Despite promise as a non-leaching antimicrobial polymer in dentistry, the impact of dimethylaminododecyl methacrylate (DMADDM) on soft liner performance remains unexplored. This study aimed to evaluate the effect of integrating different concentrations of DMADDM to cold cure acrylic resin soft liner, on its antimicrobial activity, cytotoxicity, and physical properties. The same properties were compared to a conventional commercially available denture soft liner. The study employed a control group (conventional soft liner) and three test groups containing 3.3%, 6.6%, and 10% (total mass fraction) DMADDM, respectively. Antimicrobial activity against Candida albicans and Streptococcus mutans was assessed through colony counts and biofilm biomass. Cytotoxicity was evaluated using an oral epithelial cell line. Additionally, wettability and hardness were measured to assess physical properties. Incorporation of DMADDM significantly reduced Candida albicans and Streptococcus mutans counts, and biofilm biomass, compared to the control. Additionally, DMADDM improved the soft liner's wettability and mitigated long-term hardness increase. In conclusion, DMADDM holds promise in enhancing soft liner performance. However, careful selection of its optimum concentration is crucial to ensure both safety and efficacy for future clinical use.
软性义齿衬里有限，如寿命短和微生物积累增加。尽管作为牙科非浸出性抗菌聚合物具有潜力，但二甲氨基十二烷基甲基丙烯酸酯 (DMADDM) 对软性衬里性能的影响仍未得到探索。本研究旨在评估将不同浓度的 DMADDM 整合到冷固化丙烯酸树脂软性衬里中对其抗菌活性、细胞毒性和物理性能的影响。并将这些性能与传统的商业可用的义齿软性衬里进行比较。该研究采用对照组（传统软性衬里）和三个实验组，分别含有 3.3%、6.6%和 10%（总质量分数）的 DMADDM。通过菌落计数和生物膜生物量评估对白色念珠菌和变形链球菌的抗菌活性。使用口腔上皮细胞系评估细胞毒性。此外，还测量了润湿性和硬度来评估物理性能。与对照组相比，DMADDM 的掺入显著降低了白色念珠菌和变形链球菌的数量和生物膜生物量。此外，DMADDM 提高了软性衬里的润湿性，并减轻了长期硬度增加。总之，DMADDM 有望提高软性衬里的性能。然而，为了确保未来临床应用的安全性和有效性，仔细选择其最佳浓度至关重要。
