Boronat-Echeverría Nuria, Aguirre-Mariscal Héctor, Carmolinga-Ponce Margarita, Sevilla-Delgado Yolanda, Miceli-Flores Ricardo, Kennedy-Padilla Aisha, Mejía-Aranguré Juan Manuel
Department of Pediatric Otorhinolaryngology, High Specialty Medical Care Unit of the Pediatric Hospital of the Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Av Cuauhtemoc 330, México City 06720, Mexico.
Medical Research Unit Infectious and Parasitic Diseases, High Specialty Medical Care Unit of the Pediatric Hospital of the Centro Médico Nacional Siglo XXI, Av Cuauhtemoc 330, México City 06720, Mexico.
Int J Pediatr Otorhinolaryngol. 2016 Aug;87:126-9. doi: 10.1016/j.ijporl.2016.06.023. Epub 2016 Jun 7.
The study aimed to demonstrate Helicobacter pylori presence in otitis media with effusion (OME) and its association with symptomatology of gastroesophageal reflux disease (GERD).
In a cohort study, 69 effusions were collected during tympanostomy tube insertion for H. pylori detection using PCR and ELISA. Validated questionnaires were performed according to age for clinical diagnosis of GERD; chi-square ×2 statistical analysis was made.
Eight of the 69 ear effusions (5.7%) were positive for H. pylori detection using ELISA. Two patients (2.9%) had positive results for H. pylori detection using ELISA and PCR. These eight patients had positive results too in GERD questionnaires. None of the patients with negative/suspect questionnaires had positive results for H. pylori. We found statistical association between the results of ELISA, PCR and questionnaires (×2, p = 0.001).
The H. pylori presence in effusions varies widely, in our population the frequency was lower than other reports. We found strong association between H. pylori in effusions and positive GERD questionnaires. The bacterium role in OME chronicity is not clear, but this study supports the GERD participation in OME pathogenesis.
本研究旨在证明分泌性中耳炎（OME）中幽门螺杆菌的存在及其与胃食管反流病（GERD）症状的关联。
在一项队列研究中，在鼓膜置管插入期间收集了69份积液，用于使用PCR和ELISA检测幽门螺杆菌。根据年龄进行经过验证的问卷调查以进行GERD的临床诊断；进行卡方×2统计分析。
69份耳积液中有8份（5.7%）使用ELISA检测幽门螺杆菌呈阳性。2例患者（2.9%）使用ELISA和PCR检测幽门螺杆菌呈阳性结果。这8例患者在GERD问卷调查中也呈阳性结果。问卷调查结果为阴性/可疑的患者中，无一例幽门螺杆菌检测呈阳性。我们发现ELISA、PCR结果与问卷调查之间存在统计学关联（×2，p = 0.001）。
积液中幽门螺杆菌的存在差异很大，在我们的人群中该频率低于其他报告。我们发现积液中的幽门螺杆菌与GERD问卷调查呈阳性之间存在密切关联。该细菌在OME慢性化中的作用尚不清楚，但本研究支持GERD参与OME发病机制。
