Välimäki Iiro A, Vuorimaa Timo, Ahotupa Markku, Vasankari Tommi J
Department of Health and Exercise, Paavo Nurmi Center, University of Turku, Kiinanmyllynkatu 10, 20520, Turku, Finland.
Haaga-Helia University of Applied Sciences, Vierumäki, Finland.
Eur J Appl Physiol. 2016 Sep;116(9):1683-91. doi: 10.1007/s00421-016-3422-y. Epub 2016 Jul 1.
Physical exercise has cardioprotective functions, which have been partly linked to high-density lipoprotein (HDL), and its functions. We studied the effects of endogenous oxidative stress, induced by acute exhaustive physical exercise, on concentration of oxidized HDL lipids.
Twenty-four male national top-level endurance runners, 12 middle-distance runners and 12 marathon runners performed a maximal run on a treadmill until exhaustion. We analyzed concentrations of oxidized HDL (oxHDLlipids) and LDL lipids (oxLDLlipids), serum antioxidant potential (TRAP), paraoxonase activity and malondialdehyde. Venous blood samples were taken before, immediately, 15 and 90 min after exercise.
Immediately after the treadmill run the concentration of oxHDLlipids was increased by 24 % (p < 0.01). Simultaneously, the ratio of oxHDLlipids to oxLDLlipids increased by 55 % and the oxLDLlipids levels decreased by 19 % (p < 0.001), while serum malondialdehyde and TRAP increased by 54 % (p < 0.001) and 29 % (p < 0.01), respectively. After the 90 min recovery the concentration of oxHDLlipids was decreased towards the pre-exercise level, but that of oxLDLlipids remained decreased below pre-exercise values (p < 0.001). The change in oxLDLlipids after the run correlated positively with VO2max (r = 0.67, p < 0.001) and negatively with the change in paraoxonase activity (r = -0.47, p < 0.05).
We conclude that acute exhaustive physical exercise increased the concentration of oxHDLlipids and decreased that of oxLDLlipids and the ratio of oxLDLlipids to oxHDLlipids, which suggests that during physical exercise HDL has an active role in the removal of lipid peroxides.
体育锻炼具有心脏保护功能，这部分与高密度脂蛋白（HDL）及其功能有关。我们研究了急性力竭性体育锻炼诱导的内源性氧化应激对氧化HDL脂质浓度的影响。
24名男性国家顶级耐力跑运动员、12名中长跑运动员和12名马拉松运动员在跑步机上进行最大强度跑步直至力竭。我们分析了氧化HDL（oxHDL脂质）和低密度脂蛋白脂质（oxLDL脂质）的浓度、血清抗氧化能力（TRAP）、对氧磷酶活性和丙二醛。在运动前、运动后即刻、15分钟和90分钟采集静脉血样。
跑步机跑步后即刻，oxHDL脂质浓度增加了24%（p<0.01）。同时，oxHDL脂质与oxLDL脂质的比率增加了55%，oxLDL脂质水平下降了19%（p<0.001），而血清丙二醛和TRAP分别增加了54%（p<0.001）和29%（p<0.01）。90分钟恢复后，oxHDL脂质浓度降至运动前水平，但oxLDL脂质浓度仍低于运动前值（p<0.001）。跑步后oxLDL脂质的变化与最大摄氧量呈正相关（r=0.67，p<0.001），与对氧磷酶活性的变化呈负相关（r=-0.47，p<0.05）。
我们得出结论，急性力竭性体育锻炼增加了oxHDL脂质的浓度，降低了oxLDL脂质的浓度以及oxLDL脂质与oxHDL脂质的比率，这表明在体育锻炼期间HDL在清除脂质过氧化物方面发挥着积极作用。
