Filatov A V, Bachurin P S, Markova N A, Surnakova N E
Eksp Onkol. 1989;11(2):28-32.
Monoclonal antibodies (McAb) LT1, LT2, LT4, LT6, LT7, LT8, LB21 and LDR to surface antigens of human lymphocytes have been obtained. Basing on the data concerning the distribution of McAb reactivity with cells from different organs and tissues, and with tumour cell lines, on experiments on the mutual blockage and comodulation of antigens, on two-colour immunofluorescence and determination of the molecular weight of the detected antigens a conclusion is made that the McAb obtained react with CD5, CD2, CD4, CD6, CD7, CD8, CD21 and HLA-DR antigens, respectively. McAb LT1 and Leu1, as well as LT2 and Leu5b in pairs blocked each other completely. The determinant recognized by McAb LT8 is more completely overlapped with the epitope, reacting with McAb OKT8. The McAb LT4 binding site is only partially overlapped with the McAb Leu3 site of recognition and practically is not bound to the epitope recognized by McAb OKT4.
已获得针对人淋巴细胞表面抗原的单克隆抗体（McAb）LT1、LT2、LT4、LT6、LT7、LT8、LB21和LDR。根据有关McAb与来自不同器官和组织的细胞以及肿瘤细胞系的反应性分布的数据，基于抗原相互阻断和共调节的实验，基于双色免疫荧光以及所检测抗原分子量的测定，得出结论：所获得的McAb分别与CD5、CD2、CD4、CD6、CD7、CD8、CD21和HLA - DR抗原发生反应。McAb LT1和Leu1以及LT2和Leu5b成对地完全相互阻断。McAb LT8识别的决定簇与表位更完全重叠，该表位与McAb OKT8发生反应。McAb LT4结合位点仅部分与McAb Leu3识别位点重叠，并且实际上不与McAb OKT4识别的表位结合。
