Webster Claire L, Longdon Ben, Lewis Samuel H, Obbard Darren J
Institute of Evolutionary Biology, The University of Edinburgh, Edinburgh, UK.; Evolution, behaviour and environment, School of Life Sciences, University of Sussex, Brighton, UK.
Department of Genetics, University of Cambridge, Cambridge, UK.
Evol Bioinform Online. 2016 Jun 20;12(Suppl 2):13-25. doi: 10.4137/EBO.S39454. eCollection 2016.
Drosophila melanogaster is an important laboratory model for studies of antiviral immunity in invertebrates, and Drosophila species provide a valuable system to study virus host range and host switching. Here, we use metagenomic RNA sequencing of about 1600 adult flies to discover 25 new RNA viruses associated with six different drosophilid hosts in the wild. We also provide a comprehensive listing of viruses previously reported from the Drosophilidae. The new viruses include Iflaviruses, Rhabdoviruses, Nodaviruses, and Reoviruses, and members of unclassified lineages distantly related to Negeviruses, Sobemoviruses, Poleroviruses, Flaviviridae, and Tombusviridae. Among these are close relatives of Drosophila X virus and Flock House virus, which we find in association with wild Drosophila immigrans. These two viruses are widely used in experimental studies but have not been previously reported to naturally infect Drosophila. Although we detect no new DNA viruses, in D. immigrans and Drosophila obscura, we identify sequences very closely related to Armadillidium vulgare iridescent virus (Invertebrate iridescent virus 31), bringing the total number of DNA viruses found in the Drosophilidae to three.
黑腹果蝇是研究无脊椎动物抗病毒免疫的重要实验室模型，果蝇物种为研究病毒宿主范围和宿主转换提供了一个有价值的系统。在这里，我们对约1600只成年果蝇进行宏基因组RNA测序，以发现25种与野生六种不同果蝇宿主相关的新RNA病毒。我们还提供了先前从果蝇科报道的病毒的全面列表。新病毒包括虹彩病毒、弹状病毒、诺达病毒和呼肠孤病毒，以及与内盖夫病毒、南方菜豆花叶病毒、马铃薯卷叶病毒、黄病毒科和番茄丛矮病毒科远缘相关的未分类谱系成员。其中有果蝇X病毒和 flock House病毒的近亲，我们发现它们与野生黑腹果蝇有关。这两种病毒在实验研究中被广泛使用，但以前尚未报道过它们能自然感染果蝇。虽然我们没有检测到新的DNA病毒，但在黑腹果蝇和暗果蝇中，我们鉴定出与鼠妇虹彩病毒（无脊椎动物虹彩病毒31）非常密切相关的序列，使在果蝇科中发现的DNA病毒总数达到三种。
