Guimarães Lilian de Oliveira, Bahia Santana Lobato, Ribeiro Geovani de Oliveira, Ramos Endrya do Socorro Foro, Villanova Fabiola, Dos Santos Morais Vanessa, Telles-de-Deus Juliana, Helfstein Vanessa Christe, Santos Jesus Maia Dos, Pandey Ramendra Pati, Deng Xutao, Delwart Eric, Camargo-Neves Vera Lucia Fonseca de, da Costa Antonio Charlys, Kirchgatter Karin, Leal Élcio
Área Técnica de Doenças Vinculadas a Vetores e Hospedeiros Intermediários, Instituto Pasteur, São Paulo 01027-000, SP, Brazil.
Institute of Biological Sciences, Federal University of Pará, Belem 66077-830, PA, Brazil.
Microorganisms. 2024 Aug 23;12(9):1749. doi: 10.3390/microorganisms12091749.
Metagenomic studies of mosquito viromes demonstrated a more diverse composition than just an exclusive composition of pathogenic arboviruses transmitted to humans. In our study, the virome of 866 female mosquitoes collected throughout 2020 at the São Paulo Zoo, located in the city of São Paulo/SP-Brazil, was obtained. Specifically, in this paper, we describe a new virus found by viral RNA extraction and next-generation MiSeq sequencing of a group of 23 specimens of (.) . The complete genome with a length of 9709 nucleotides was characterized by a positive orientation and a single strand, with a single large ORF, which encodes a polyprotein of 2987 amino acids. The phylogenetic analysis showed an association with the viral family and the realm. We carried out comparisons with translated sequences of the capsid regions of other iflavirus, and the identities in relation to our sequence were below the minimum limit of 90%, indicating that possibly it is a new species of iflavirus. Our findings contribute to expanding knowledge of virome composition among mosquito species in Brazil and globally. Moreover, we provide a viral genome reference specific to this geographic region and family of mosquitoes. This resource facilitates future in silico recognition and assembly of viral genomes within metagenomic datasets.
对蚊子病毒组的宏基因组学研究表明，其组成比仅仅是传播给人类的致病性虫媒病毒的单一组成更为多样。在我们的研究中，获得了2020年全年在巴西圣保罗市圣保罗动物园采集的866只雌性蚊子的病毒组。具体而言，在本文中，我们描述了通过对一组23个样本进行病毒RNA提取和下一代MiSeq测序发现的一种新病毒。该病毒的完整基因组长度为9709个核苷酸，具有正向单链结构，有一个单一的大开放阅读框，编码一个由2987个氨基酸组成的多聚蛋白。系统发育分析表明它与病毒科和病毒界有关联。我们将其与其他黄病毒科病毒衣壳区域的翻译序列进行了比较，与我们序列的同一性低于90%的最低限度，这表明它可能是黄病毒科的一个新物种。我们的研究结果有助于扩大对巴西乃至全球蚊子物种病毒组组成的认识。此外，我们提供了针对该地理区域和蚊子科的病毒基因组参考。这一资源便于未来在宏基因组数据集中对病毒基因组进行计算机识别和组装。
