Soybean Research Institute of Nanjing Agricultural University/National Center for Soybean Improvement/National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing, 210095, Jiangsu, China.
Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, No. 50, Zhongling Street, Nanjing, 210014, Jiangsu, China.
BMC Genom Data. 2021 Sep 16;22(1):34. doi: 10.1186/s12863-021-00989-w.
High salinity is a devastating abiotic stresses for crops. To understand the molecular basis of salinity stress in yardlong bean (Vigna unguiculata ssp. sesquipedalis), and to develop robust markers for improving this trait in germplasm, whole transcriptome RNA sequencing (RNA-seq) was conducted to compare the salt-tolerant variety Suzi 41 and salt-sensitive variety Sujiang 1419 under normal and salt stress conditions.
Compared with controls, 417 differentially expressed genes (DEGs) were identified under exposure to high salinity, including 42 up- and 11 down-regulated DEGs in salt-tolerant Suzi 41 and 186 up- and 197 down-regulated genes in salt-sensitive Sujiang 1419, validated by qRT-PCR. DEGs were enriched in "Glycolysis/Gluconeogenesis" (ko00010), "Cutin, suberine and wax biosynthesis" (ko00073), and "phenylpropanoid biosynthesis" (ko00940) in Sujiang 1419, although "cysteine/methionine metabolism" (ko00270) was the only pathway significantly enriched in salt-tolerant Suzi 41. Notably, AP2/ERF, LR48, WRKY, and bHLH family transcription factors (TFs) were up-regulated under high salt conditions. Genetic diversity analysis of 84 yardlong bean accessions using 26 InDel markers developed here could distinguish salt-tolerant and salt-sensitive varieties.
These findings show a limited set of DEGs, primarily TFs, respond to salinity stress in V. unguiculata, and that these InDels associated with salt-inducible loci are reliable for diversity analysis.
高盐度是一种对作物具有破坏性的非生物胁迫。为了了解豇豆（Vignaunguiculatassp.sesquipedalis）盐胁迫的分子基础，并开发用于改良种质资源的稳健标记，对耐盐品种 Suzi41 和盐敏感品种 Sujiang1419 在正常和盐胁迫条件下进行了全转录组 RNA 测序（RNA-seq）。
与对照相比，在高盐胁迫下鉴定出 417 个差异表达基因（DEGs），其中耐盐 Suzi41 中有 42 个上调和 11 个下调的 DEGs，盐敏感 Sujiang1419 中有 186 个上调和 197 个下调的 DEGs，通过 qRT-PCR 验证。在 Sujiang1419 中，DEGs 富集在“糖酵解/糖异生”（ko00010）、“角质、栓皮和蜡生物合成”（ko00073）和“苯丙素生物合成”（ko00940）途径中，尽管“半胱氨酸/蛋氨酸代谢”（ko00270）是耐盐 Suzi41 中唯一显著富集的途径。值得注意的是，AP2/ERF、LR48、WRKY 和 bHLH 家族转录因子（TFs）在高盐条件下上调。使用这里开发的 26 个 InDel 标记对 84 个豇豆品种进行遗传多样性分析，可以区分耐盐和盐敏感品种。
这些发现表明，一组有限的 DEGs，主要是 TFs，对 V.unguiculata 的盐胁迫有反应，并且这些与盐诱导基因座相关的 InDels 可用于可靠的多样性分析。
