骨髓源干细胞在急性放射综合征中的治疗作用。

The healing effect of bone marrow-derived stem cells in acute radiation syndrome.

作者信息

Mortazavi Seyed Mohammad Javad, Shekoohi-Shooli Fatemeh, Aghamir Seyed Mahmood Reza, Mehrabani Davood, Dehghanian Amirreza, Zare Shahrokh, Mosleh-Shirazi Mohammad Amin

机构信息

Seyed Mohammad Javad Mortazavi, Ionizing and Non-ionizing Radiation Protection Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

Fatemeh Shekoohi-Shooli , Radiology and Radiotherapy Department, Shahid Beheshti University of Medical Science, Tehran, Iran.

出版信息

Pak J Med Sci. 2016 May-Jun;32(3):646-51. doi: 10.12669/pjms.323.9895.

OBJECTIVES

To determine the effect of bone marrow-derived mesenchymal stem cells (BMSCs) on regeneration of bone marrow and intestinal tissue and survival rate in experimental mice with acute radiation syndrome (ARS).

METHODS

Forty mice were randomly divided into two equal groups of A receiving no BMSC transplantation and B receiving BMSCs. BMSCs were isolated from the bone marrow and cultured in DMEM media. Both groups were irradiated with 10 Gy (dose rate 0.28 Gy/ min) (60)CO during 35 minutes with a field size of 35×35 for all the body area. Twenty-four hours after γ irradiation, 150×10(3) cells of passage 5 in 150 µl medium were injected intravenously into the tail. Animals were euthanized one and two weeks after cell transplantation. They were evaluated histologically for any changes in bone marrow and intestinal tissues. The survival rate in mice were also determined.

RESULTS

A significant increase for bone marrow cell count and survival rate were observed in group B in comparison to group A. Histological findings denoted to a healing in sample tissues.

CONCLUSION

BMSCs could significantly reduce the side effects of ARS and increase the survival rate and healing in injured tissue. As such their transplantation may open a window in treatment of patients with ARS.

目的

确定骨髓间充质干细胞（BMSCs）对急性放射综合征（ARS）实验小鼠骨髓和肠道组织再生及存活率的影响。

方法

40只小鼠随机分为两组，每组20只。A组不进行BMSC移植，B组接受BMSC移植。从骨髓中分离BMSCs并在DMEM培养基中培养。两组均在35分钟内接受10 Gy（剂量率0.28 Gy/min）的（60）Co全身照射，照射野大小为35×35。γ射线照射24小时后，将150×10（3）个第5代细胞悬浮于150 μl培养基中经尾静脉注射。细胞移植后1周和2周对动物实施安乐死。对骨髓和肠道组织的任何变化进行组织学评估。同时测定小鼠的存活率。