Ricard Clément, Lamasse Lisa, Jaouen Alexandre, Rougon Geneviève, Debarbieux Franck
Institut des Neurosciences de la Timone, Marseille, Aix-Marseille Université and CNRS UMR7289, France; Centre Européen de Recherche en Imagerie Médicale, Aix-Marseille Université, Marseille, France; Service de Pharmacie, Assistance Publique, Hôpitaux de Marseille, Marseille, France; Centre de Recherche en Oncobiologie et Oncopharmacologie, INSERM UMR911 and Aix-Marseille Université, Marseille, France; These authors contributed equally to this work.
Institut des Neurosciences de la Timone, Marseille, Aix-Marseille Université and CNRS UMR7289, France; Centre Européen de Recherche en Imagerie Médicale, Aix-Marseille Université, Marseille, France; These authors contributed equally to this work.
Biomed Opt Express. 2016 May 24;7(6):2362-72. doi: 10.1364/BOE.7.002362. eCollection 2016 Jun 1.
Simultaneous imaging of different cell types and structures in the mouse central nervous system (CNS) by intravital two-photon microscopy requires the characterization of fluorophores and advances in approaches to visualize them. We describe the use of a two-photon infrared illumination generated by an optical parametric oscillator (OPO) on quantum-dots 655 (QD655) nanocrystals to improve resolution of the vasculature deeper in the mouse brain both in healthy and pathological conditions. Moreover, QD655 signal can be unmixed from the DsRed2, CFP, EGFP and EYFP fluorescent proteins, which enhances the panel of multi-parametric correlative investigations both in the cortex and the spinal cord.
通过活体双光子显微镜对小鼠中枢神经系统（CNS）中的不同细胞类型和结构进行同步成像，需要对荧光团进行表征并改进可视化方法。我们描述了使用光学参量振荡器（OPO）产生的双光子红外照明照射量子点655（QD655）纳米晶体，以提高在健康和病理条件下小鼠脑深部脉管系统的分辨率。此外，QD655信号可以与DsRed2、CFP、EGFP和EYFP荧光蛋白的信号分离，这增强了在皮质和脊髓中进行多参数相关研究的能力。
