Division of Dermatology, Department of Internal Related, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuou-ku, Kobe 650-0017, Japan.
Kindai University Atomic Energy Research Institute, 3-4-1 Kowakae, Higashiosaka City, Osaka 577-8502, Japan.
Sci Rep. 2016 Jul 5;6:29233. doi: 10.1038/srep29233.
Ultraviolet (UV) radiation induces a variety of biological effects, including DNA damage response and cell signaling pathways. We performed transcriptome analysis using microarray in human primary cultured fibroblasts irradiated with UV-C (0.5 or 5 J/m(2)) and harvested at 4 or 12 h following UV exposure. All transcript data were analyzed by comparison with the corresponding results in non-irradiated (control) cells. The number of genes with significantly altered expression (≥2-fold difference relative to the control) is higher in the sample irradiated with high dose of UV, suggesting that gene expression was UV dose-dependent. Pathway analysis on the upregulated genes at 12 h indicates that the expression of some cell cycle-related genes was predominantly induced irrespective of UV-dose. Interestingly, almost all the genes with significant altered expression were cell cycle-related genes designated as 'Mitotic Genes', which function in the spindle assembly checkpoint. Therefore, even a low dose of UV could affect the transcriptional profile.
紫外线（UV）辐射会引起多种生物效应，包括 DNA 损伤反应和细胞信号通路。我们使用微阵列在人类原代培养成纤维细胞中进行转录组分析，这些细胞经 UV-C（0.5 或 5J/m2）照射，并在 UV 暴露后 4 或 12 小时收获。所有转录数据均通过与未照射（对照）细胞的相应结果进行比较进行分析。高剂量 UV 照射组中表达明显改变的基因数量（相对于对照增加 2 倍以上）较高，这表明基因表达与 UV 剂量有关。12 小时时上调基因的通路分析表明，一些细胞周期相关基因的表达主要被诱导，而与 UV 剂量无关。有趣的是，几乎所有表达明显改变的基因都是细胞周期相关基因，被指定为“有丝分裂基因”，它们在纺锤体组装检查点中发挥作用。因此，即使是低剂量的 UV 也可能影响转录谱。
