Fujimoto T, Hata J, Yokoyama S, Mitomi T
Department of Paediatric Surgery, Tokai University, School of Medicine, Isehara, Japan.
J Pediatr Surg. 1989 Jun;24(6):550-6. doi: 10.1016/s0022-3468(89)80504-4.
Immunocytochemical studies on the human embryo were made using antineuronal cell antibody and a panel of anti-extracellular matrix protein antibodies such as fibronectin, laminine, collagen type IV, and hyaluronic acid. All the enteric ganglia are shown to be from a single, vagal neural crest source, although the recent dual gradient migration theory of neural-crest-derived cells in the gut can be challenged. Neural-crest-derived cells first appear in the mesenchyme of the developing esophagus at 4 weeks, and then migrate down along the gut in a craniocaudal direction. The observed distribution of fibronectin and hyaluronic acid indicates the presence of these matrices providing a migration pathway for neural-crest-derived cells in the developing gut. The appearance of neural-crest-derived cells in the gut is always preceded by the appearance of these matrices. On the other hand, substrate or laminine and collagen type IV appears to promote outgrowth of neurites from settled neural-crest-derived cells and their maturation. The distribution of these matrices within the pathway seems consistent with their role in navigating the neural-crest-derived cells toward their final destination. Enteric neurogenesis is dependent on these matrices, and their alteration in early embryonal stage may be a significant factor in the pathogenesis of Hirschsprung's disease.
利用抗神经元细胞抗体以及一组抗细胞外基质蛋白抗体（如纤连蛋白、层粘连蛋白、IV型胶原和透明质酸）对人类胚胎进行了免疫细胞化学研究。尽管肠道中神经嵴衍生细胞最近的双梯度迁移理论可能受到挑战，但所有肠神经节均显示来自单一的迷走神经嵴来源。神经嵴衍生细胞在4周时首次出现在发育中的食管间充质中，然后沿肠道向头尾方向迁移。观察到的纤连蛋白和透明质酸的分布表明，这些基质的存在为发育中的肠道中神经嵴衍生细胞提供了迁移途径。神经嵴衍生细胞在肠道中的出现总是先于这些基质的出现。另一方面，底物或层粘连蛋白和IV型胶原似乎促进定居的神经嵴衍生细胞的神经突生长及其成熟。这些基质在途径中的分布似乎与其引导神经嵴衍生细胞到达最终目的地的作用一致。肠神经发生依赖于这些基质，它们在胚胎早期的改变可能是先天性巨结肠病发病机制中的一个重要因素。
