Donato Celeste M, Manuelpillai Nicholas M, Cowley Daniel, Roczo-Farkas Susie, Buttery Jim P, Crawford Nigel W, Kirkwood Carl D
Enteric Virus Group, Murdoch Childrens Research Institute, Melbourne, Victoria, Australia; Department of Microbiology, La Trobe University, Melbourne, Victoria, Australia.
Enteric Virus Group, Murdoch Childrens Research Institute, Melbourne, Victoria, Australia; School of Medicine, University of Notre Dame, Sydney, New South Wales, Australia.
Infect Genet Evol. 2014 Jul;25:97-109. doi: 10.1016/j.meegid.2014.04.009. Epub 2014 Apr 26.
A genotype G3P[14] rotavirus strain was identified in a 12year old child presenting to the Emergency Department of the Royal Children's Hospital, Melbourne, with gastroenteritis. G3P[14] strains have been previously identified in rabbits in Japan, China, the USA and Italy and a single lapine-like strain from a child in Belgium. Full genome sequence analysis of RVA/Human-wt/AUS/RCH272/2012/G3P[14] (RCH272) revealed that the strain contained the novel genome constellation G3-P[14]-I2-R3-C3-M3-A9-N2-T6-E2-H3. The genome was genetically divergent to previously characterized lapine viruses and the genes were distantly related to a range of human bovine-like strains and animal strains of bovine, bat and canine/feline characteristics. The VP4, VP6, NSP2, NSP3, NSP4 and NSP5 genes of RCH272 clustered within bovine lineages in the phylogenetic analysis and shared moderate genetic similarity with an Australian bovine-like human strain RVA/Human-tc/AUS/MG6/1993/G6P[14]. Bayesian coalescent analysis suggested these genes of RCH272 and RVA/Human-tc/AUS/MG6/1993/G6P[14] were derived from a population of relatively homogenous bovine-like ancestral strains circulating between 1943 and 1989. The VP7, VP1, VP2 and NSP1 genes shared moderate genetic similarity with the Chinese strain RVA/Bat-tc/CHN/MSLH14/2011/G3P[3] and the VP3 gene clustered within a lineage comprised of canine and feline strains. This strain may represent the direct transmission from an unknown host species or be derived via multiple reassortment events between strains originating from various species. The patient lived in a household containing domesticated cats and dogs and in close proximity to a colony of Gray-headed Flying-foxes. However, without screening numerous animal populations it is not possible to determine the origins of this strain.
在墨尔本皇家儿童医院急诊科就诊的一名12岁患肠胃炎儿童体内，鉴定出一株G3P[14]基因型轮状病毒。此前在日本、中国、美国和意大利的兔子中以及比利时一名儿童体内鉴定出G3P[14]毒株。对RVA/Human-wt/AUS/RCH272/2012/G3P[14]（RCH272）进行全基因组序列分析发现，该毒株含有新型基因组组合G3-P[14]-I2-R3-C3-M3-A9-N2-T6-E2-H3。该基因组在遗传上与先前鉴定的兔病毒不同，其基因与一系列具有人牛样特征的毒株以及牛、蝙蝠和犬/猫特征的动物毒株关系较远。在系统发育分析中，RCH272的VP4、VP6、NSP2、NSP3、NSP4和NSP5基因聚集在牛系中，与澳大利亚人牛样毒株RVA/Human-tc/AUS/MG6/1993/G6P[14]具有中等程度的遗传相似性。贝叶斯合并分析表明，RCH272和RVA/Human-tc/AUS/MG6/1993/G6P[14]的这些基因源自1943年至1犹989年间循环的相对同质的牛样祖先毒株群体。VP7、VP1、VP2和NSP1基因与中国毒株RVA/Bat-tc/CHN/MSLH14/2011/G3P[犹3]具有中等程度的遗传相似性，VP3基因聚集在一个由犬和猫毒株组成的谱系中。该毒株可能代表来自未知宿主物种的直接传播，或者是通过源自不同物种的毒株之间的多次重配事件产生的。该患者生活在一个养有宠物猫和狗的家庭中且紧邻一群灰头狐蝠。然而，在未对大量动物群体进行筛查的情况下，无法确定该毒株的来源。
