对吸烟农药喷洒工淋巴细胞中姐妹染色单体交换、细胞动力学和有丝分裂指数的分析。

Analysis of sister-chromatid exchanges, cell kinetics and mitotic index in lymphocytes of smoking pesticide sprayers.

作者信息

Rupa D S, Reddy P P, Reddi O S

机构信息

Institute of Genetics, Osmania University, Andhra Pradesh, India.

出版信息

Mutat Res. 1989 Jun;223(2):253-8. doi: 10.1016/0165-1218(89)90053-0.

DOI:10.1016/0165-1218(89)90053-0

PMID:2739682

Abstract

Whole blood of 50 smokers who were exposed to pesticides was set up in RPMI 1640 medium, and observed for sister-chromatid exchanges (SCEs), cell kinetics (CK) and mitotic index (MI). As controls, blood samples were collected from 20 non-smokers (control I) and 27 smokers (control II) who were not exposed to pesticides. A significant increase in SCEs was observed as the duration of exposure increased. The frequency of M1 metaphases increased significantly whereas M2 and M3+ metaphases decreased in the exposed group. The mitotic index increased in control II and in the exposed population while it showed a decrease at 11-25 years' exposure.

摘要

将50名接触农药的吸烟者的全血置于RPMI 1640培养基中，观察其姐妹染色单体交换（SCE）、细胞动力学（CK）和有丝分裂指数（MI）。作为对照，采集了20名未接触农药的非吸烟者（对照I）和27名未接触农药的吸烟者（对照II）的血样。随着接触时间的增加，观察到SCE显著增加。暴露组中M1中期的频率显著增加，而M2和M3 +中期减少。对照II和暴露人群的有丝分裂指数增加，而在暴露11 - 25年时则呈下降趋势。