Vestergaard P
Acta Endocrinol Suppl (Copenh). 1978;217:96-120.
Complete hydrolysis of known conjugates of neutral steroids in urine requires a combination of solvolysis and enzymatic hydrolysis. Procedures are established for optimal yield in the enzymatic part of the hydrolysis using different preparations of beta-glucuronidase. The effect of temperature and the use of conjugate extraction in the hydrolytic procedure is explored. It is stressed that relatively high concentrations of enzyme are necessary to insure full hydrolysis of a few conjugates that are particularly difficult to split by enzymatic hydrolysis.
尿液中已知中性类固醇结合物的完全水解需要溶剂解和酶解相结合。已建立了使用不同制剂的β-葡萄糖醛酸酶在水解的酶解部分获得最佳产率的方法。探索了温度的影响以及在水解过程中使用结合物提取的情况。需要强调的是，为确保某些特别难以通过酶解拆分的结合物完全水解，需要相对高浓度的酶。
