Gupta A, Anand A C, Chumber S K, Sashindran V K, Patrikar S R
Internee Medical Officer, Military Hospital, Secunderabad.
Dy DGMS (P) O/o DGMS (Army), AG's Branch, IHQ of MOD (Army), New Delhi.
Med J Armed Forces India. 2007 Oct;63(4):334-6. doi: 10.1016/S0377-1237(07)80009-8. Epub 2011 Jul 21.
Use of protective footwear before entering the intensive care units is enforced with the assumption that it lowers the incidence of bacterial floor colonization. The present study was carried out to find the efficacy of protective footwear on bacterial floor colonization.
The study was carried out in the intensive care unit of a tertiary care hospital. The study was divided into two phases of two weeks each, phase I with and phase II without use of protective footwear. Samples were taken at six different sites namely footwear exchange area; visitors /staff route; partitioned patient cubicle; central monitoring area; open patient cubicle and scrub up areas. Floor samples were taken at 0600, 1100, 1700 and 2200 hours and air samples at 0600 and 1700 hours. Bacteria were identified and colony forming units (cfu) measured from floor and colony forming units/metre(3) (cfu/m3) from air sample cultures.
A total of 9521 bacterial colony forming units were isolated from 192 samples in phase I from the floor samples and 9971cfu from 192 samples in phase II. From 96 air samples in each phase, a mean of 262 cfu/m(3) in phase I and 220cfu/m(3) in phase II were isolated. The difference between the two phases was statistically not significant (p value > 0.05 for both).
Floor and air colony counts showed no significant difference in the two phases with and without protective footwear. Protective footwear had no significant impact on bacterial contamination of floors.
进入重症监护病房前使用防护鞋是基于其能降低细菌在地面定植发生率的假设而实施的。本研究旨在探究防护鞋对细菌在地面定植的效果。
本研究在一家三级医院的重症监护病房进行。研究分为两个为期两周的阶段，第一阶段使用防护鞋，第二阶段不使用防护鞋。在六个不同地点采集样本，即鞋类更换区；访客/工作人员通道；分隔的患者隔间；中央监测区；开放式患者隔间和刷手区。地面样本在06:00、11:00、17:00和22:00采集，空气样本在06:00和17:00采集。对细菌进行鉴定，并测量地面样本的菌落形成单位（cfu）以及空气样本培养物的每立方米菌落形成单位（cfu/m³）。
在第一阶段，从192个地面样本中总共分离出9521个细菌菌落形成单位，在第二阶段从192个样本中分离出9971 cfu。在每个阶段的96个空气样本中，第一阶段平均分离出262 cfu/m³，第二阶段平均分离出220 cfu/m³。两个阶段之间的差异在统计学上不显著（两者p值均>0.05）。
在使用和不使用防护鞋的两个阶段，地面和空气菌落计数均无显著差异。防护鞋对地面细菌污染没有显著影响。
