巴西帕拉州贝伦大都市区美洲皮肤利什曼病的生态流行病学的进一步见解。
Further insights into the eco-epidemiology of American cutaneous leishmaniasis in the Belem metropolitan region, Pará State, Brazil.
机构信息
Instituto Evandro Chagas, Laboratório de Leishmanioses 'Prof. Dr. Ralph Lainson', Seção de Parasitologia, Ananindeua, PA, Brasil.
Universidade Federal do Pará, Núcleo de Medicina Tropical, Belém, PA, Brasil.
出版信息
Rev Soc Bras Med Trop. 2020 Dec 11;53:e20200255. doi: 10.1590/0037-8682-0255-2020. eCollection 2020.
INTRODUCTION
In the Belém Metropolitan Region (BMR), Pará State, Brazil, American cutaneous leishmaniasis (ACL) is endemic; however, very little is known regarding its causative agents. Therefore, we used our standard diagnostic approach combined with an RNA polymerase II largest subunit (RNAPOIILS)-polymerase chain reaction (PCR) followed by analysis of restriction fragment length polymorphism (PCR-RFLP) to identify Leishmania spp. ACL agents in this region.
METHODS
Thirty-two Leishmania spp. isolates from patients with ACL in the BMR during 1995-2018 were analyzed. Leishmania spp. DNA samples were amplified using the primers RPOR2/RPOF2, and the 615-bp PCR products were subjected to enzymatic digestion using TspRI and HgaI endonucleases.
RESULTS
ACL etiological agents in the BMR comprised Leishmania (Viannia) lindenbergi (43.7%) followed by Leishmania (Viannia) lainsoni (34.4%), Leishmania (Leishmania) amazonensis (12.5%), and Leishmania (Viannia) braziliensis (9.4%).
CONCLUSIONS
To our knowledge, the results of the study revealed for the first time that L. (V.) lindenbergi and L. (V.) lainsoni are the main ACL agents in BMR.
简介
在巴西帕拉州贝伦都会区（BMR），存在地方性美洲皮肤利什曼病（ACL）；然而，对于其病原体知之甚少。因此，我们采用标准诊断方法，结合 RNA 聚合酶 II 大亚基（RNAPOIILS）-聚合酶链反应（PCR），并对其进行限制性片段长度多态性（PCR-RFLP）分析，以确定该地区的 ACL 病原体。
方法
1995 年至 2018 年，对 BMR 中 32 例 ACL 患者的利什曼原虫进行了分析。使用引物 RPOR2/RPOF2 扩增利什曼原虫 DNA 样本，615bp 的 PCR 产物用 TspRI 和 HgaI 内切酶进行酶切。
结果
BMR 的 ACL 病原体包括利什曼原虫（Viannia）lindenbergi（43.7%），其次是利什曼原虫（Viannia）lainsoni（34.4%）、利什曼原虫（Leishmania）amazonensis（12.5%）和利什曼原虫（Viannia）braziliensis（9.4%）。
结论
据我们所知，这项研究的结果首次表明，L.（V.）lindenbergi 和 L.（V.）lainsoni 是 BMR 中主要的 ACL 病原体。
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