Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Trans R Soc Trop Med Hyg. 2011 Oct;105(10):561-7. doi: 10.1016/j.trstmh.2011.05.009. Epub 2011 Sep 9.
A molecular epidemiological study was performed using FTA card materials directly sampled from lesions of patients with cutaneous leishmaniasis (CL) in the state of Lara, Venezuela, where causative agents have been identified as Leishmania (Viannia) braziliensis and L. (Leishmania) venezuelensis in previous studies. Of the 17 patients diagnosed with CL, Leishmania spp. were successfully identified in 16 patients based on analysis of the cytochrome b gene and rRNA internal transcribed spacer sequences. Consistent with previous findings, seven of the patients were infected with L. (V.) braziliensis. However, parasites from the other nine patients were genetically identified as L. (L.) mexicana, which differed from results of previous enzymatic and antigenic analyses. These results strongly suggest that L. (L.) venezuelensis is a variant of L. (L.) mexicana and that the classification of L. (L.) venezuelensis should be reconsidered.
采用直接从委内瑞拉拉拉州皮肤利什曼病（CL）患者皮损中取样的 FTA 卡材料进行了分子流行病学研究。在先前的研究中，已确定该州的病原体为 Leishmania（Viannia）braziliensis 和 L.（Leishmania）venezuelensis。在 17 例确诊为 CL 的患者中，根据细胞色素 b 基因和 rRNA 内部转录间隔区序列分析，成功鉴定了 16 例患者的 Leishmania spp。与先前的发现一致，7 例患者感染了 L.（V.）braziliensis。然而，来自其他 9 例患者的寄生虫在基因上被鉴定为 L.（L.）mexicana，这与先前的酶和抗原分析结果不同。这些结果强烈表明 L.（L.）venezuelensis 是 L.（L.）mexicana 的变体，应重新考虑 L.（L.）venezuelensis 的分类。
