Ren Yong-Cheng, Liu Xiao-Jing, Yi Ze-Hao, Hui Feng-Li
School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, PR China.
Int J Syst Evol Microbiol. 2016 Oct;66(10):4046-4050. doi: 10.1099/ijsem.0.001308. Epub 2016 Jul 13.
Three strains of a novel species of ascomycetous yeast were isolated from the beetle species Anomala corpulenta (Scarabaeoidea) collected from the Baotianman and Funiu Mountains of China. These strains produced conjugated asci with a single coiled ascospore. Phylogenetic analysis of the combined sequences of the D1/D2 domains of the large subunit rRNA gene and internal transcribed spacer regions demonstrated that the three strains were closely related to Nematodospora valgi and an undescribed yeast strain, 13Y231. The novel strains could be differentiated from N. valgi CBS 12562T by a 1.6 % sequence divergence (9 substitutions) and from the undescribed yeast strain, 13Y231, by a 1.1 % sequence divergence (6 substitutions) in the D1/D2 sequences. The ITS sequences of these strains displayed more than 4.1 % sequence divergence (12-22 substitutions and 7-8 gaps) from their two closest relatives. Interestingly, all the three strains could ferment d-xylose to ethanol effectively, a rare property among members of the Lodderomyces clade. Therefore, a novel yeast species, Nematodosporaanomalae sp. nov., is proposed to accommodate these strains. The type strain of N.anomalae sp. nov. is NYNU 14914T (=CICC 33059T=CBS 13927T). The MycoBank number is MB 816795.
从中国宝天曼和伏牛山采集的铜绿异丽金龟（鞘翅目）中分离出三株新的子囊菌酵母。这些菌株产生具单个卷曲子囊孢子的接合子囊。对大亚基rRNA基因的D1/D2结构域和内部转录间隔区的联合序列进行系统发育分析表明，这三株菌株与瓦尔吉线虫孢菌和一株未描述的酵母菌株13Y231密切相关。在D1/D2序列中，新菌株与瓦尔吉线虫孢菌CBS 12562T的序列差异为1.6%（9个替换），与未描述的酵母菌株13Y231的序列差异为1.1%（6个替换），从而可将其区分开来。这些菌株的ITS序列与其两个最亲近的亲缘种的序列差异超过4.1%（12 - 22个替换和7 - 8个空位）。有趣的是，所有这三株菌株都能有效地将D - 木糖发酵成乙醇，这在罗地酵母属成员中是一种罕见的特性。因此，提议将这些菌株归为一个新的酵母物种，即奇异线虫孢酵母（Nematodospora anomalae sp. nov.）。奇异线虫孢酵母的模式菌株为NYNU 14914T（=CICC 33059T = CBS 13927T）。国际真菌命名数据库编号为MB 816795。
