Miljkovic Milica, Kotur-Stevuljevic Jelena, Stefanovic Aleksandra, Zeljkovic Aleksandra, Vekic Jelena, Gojkovic Tamara, Bogavac-Stanojevic Natasa, Nikolic Milan, Simic-Ogrizovic Sanja, Spasojevic-Kalimanovska Vesna, Jelic-Ivanovic Zorana
Department of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, P. Box 146, 11000, Belgrade, Serbia.
Department of Biochemistry, Faculty of Chemistry, University of Belgrade, Studentski trg 16, 11000, Belgrade, Serbia.
Int Urol Nephrol. 2016 Oct;48(10):1683-90. doi: 10.1007/s11255-016-1358-0. Epub 2016 Jul 13.
Unfavorable lipid profile is a major risk factor for cardiovascular disease in renal pathology. In this study, we compared chronic renal patients and healthy controls with different LDL phenotypes (A or B) in respect of various biochemical parameters related to cardiovascular disease.
Oxidative stress and anti-oxidative defense parameters [thiobarbituric acid-reacting substances (TBARS), total oxidative status (TOS), total anti-oxidative status (TAS), total protein sulfhydryl (-SH) groups], as well as red blood cell cholesterol distribution were assessed in 40 renal patients and 40 control subjects by standardized assays. LDL particle diameters were determined by polyacrylamide gradient gel electrophoresis. LDL particles are subdivided according to their size into large LDL A phenotype (diameter >25.5 nm) and small LDL B phenotype (diameter ≤25.5 nm).
Renal patients with LDL A phenotype had increased oxidative stress (TOS: p < 0.01, and TBARS: p < 0.001) and decreased total SH- groups (p < 0.001) compared to controls with the same LDL phenotype. A notable decrease in hemoglobin-cholesterol adduct was detected in patients with LDL A phenotype (p < 0.001) and LDL B phenotype (p < 0.05) compared with appropriate controls. LDL B phenotype was characterized with increased TBARS (p < 0.05) compared with LDL A phenotype in control group.
Increased oxidative stress, decreased anti-oxidative defense followed with unfavorable changes in hemoglobin-cholesterol binding capacity, could have important influence on cardiovascular disease risk in renal patients regardless of LDL phenotype.
不良脂质谱是肾脏疾病中心血管疾病的主要危险因素。在本研究中，我们比较了不同低密度脂蛋白（LDL）表型（A或B）的慢性肾病患者和健康对照者在与心血管疾病相关的各种生化参数方面的差异。
通过标准化检测方法，对40例肾病患者和40例对照者进行氧化应激和抗氧化防御参数[硫代巴比妥酸反应物质（TBARS）、总氧化状态（TOS）、总抗氧化状态（TAS）、总蛋白巯基（-SH）基团]以及红细胞胆固醇分布的评估。通过聚丙烯酰胺梯度凝胶电泳测定LDL颗粒直径。LDL颗粒根据其大小分为大LDL A表型（直径>25.5nm）和小LDL B表型（直径≤25.5nm）。
与具有相同LDL表型的对照者相比，具有LDL A表型的肾病患者氧化应激增加（TOS：p<0.01，TBARS：p<0.001），总SH基团减少（p<0.001）。与相应对照者相比，LDL A表型（p<0.001）和LDL B表型（p<0.05）的患者血红蛋白 - 胆固醇加合物显著降低。在对照组中，与LDL A表型相比，LDL B表型的特征是TBARS增加（p<0.05）。
氧化应激增加、抗氧化防御降低以及血红蛋白 - 胆固醇结合能力的不利变化，无论LDL表型如何，都可能对肾病患者的心血管疾病风险产生重要影响。
