Belser Jessica A, Eckert Alissa M, Tumpey Terrence M, Maines Taronna R
Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
Division of Communication Services, Office of the Associate Director for Communication, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Microbiol Mol Biol Rev. 2016 Jul 13;80(3):733-44. doi: 10.1128/MMBR.00022-16. Print 2016 Sep.
Ferrets are widely employed to study the pathogenicity, transmissibility, and tropism of influenza viruses. However, inherent variations in inoculation methods, sampling schemes, and experimental designs are often overlooked when contextualizing or aggregating data between laboratories, leading to potential confusion or misinterpretation of results. Here, we provide a comprehensive overview of parameters to consider when planning an experiment using ferrets, collecting data from the experiment, and placing results in context with previously performed studies. This review offers information that is of particular importance for researchers in the field who rely on ferret data but do not perform the experiments themselves. Furthermore, this review highlights the breadth of experimental designs and techniques currently available to study influenza viruses in this model, underscoring the wide heterogeneity of protocols currently used for ferret studies while demonstrating the wealth of information which can benefit risk assessments of emerging influenza viruses.
雪貂被广泛用于研究流感病毒的致病性、传播性和嗜性。然而，在整合或汇总不同实验室的数据时，接种方法、采样方案和实验设计中的固有差异常常被忽视，这可能导致对结果的潜在混淆或误解。在此，我们全面概述了在使用雪貂进行实验规划、从实验中收集数据以及将结果与先前进行的研究相结合时需要考虑的参数。本综述为该领域依赖雪貂数据但不亲自进行实验的研究人员提供了特别重要的信息。此外，本综述强调了目前可用于在此模型中研究流感病毒的实验设计和技术的广度，突显了当前用于雪貂研究的方案存在广泛的异质性，同时展示了可为新兴流感病毒风险评估提供益处的丰富信息。
