Qiu Jiang, Chen Yehui, Huang Gang, Zhang Zhi, Chen Lizhong, Na Ning
Division of Organ Transplantation, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
Division of Urology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, China.
Nephrology (Carlton). 2017 Oct;22(10):796-803. doi: 10.1111/nep.12851.
Long noncoding RNAs (lncRNAs) are novel intracellular noncoding ribonucleotides regulating the genome and proteome. The lncRNA activated by transforming growth factor β (TGF-β) (lncRNA-ATB) was discovered as a prognostic factor in hepatocellular carcinoma, gastric cancer, and colorectal cancer. However, little is known about the role of lncRNA-ATB in renal transplantation. This study aimed to assess lncRNA-ATB expression in renal transplant patients with acute kidney injury and explore its role in postoperative pharmaceutical immunosuppression therapy.
We detected lncRNA-ATB expression in the kidney biopsies of a cohort of 72 patients with renal allograft rejection and 36 control transplant patients. lncRNA-ATB were overexpressed from lentiviral vectors in renal cells.
We found that lncRNA-ATB was remarkably upregulated in patients with acute rejection compared with controls. Meanwhile, lncRNA-ATB could influence the kidney cell phenotypes and impact the nephrotoxicity of immunosuppressive drug.
In conclusion, lncRNA-ATB are strongly altered in patients with acute rejection and may serve as a novel biomarker of acute kidney rejection, identifying patients with acute rejection and predicting loss of kidney function.
长链非编码核糖核酸（lncRNAs）是调节基因组和蛋白质组的新型细胞内非编码核糖核苷酸。转化生长因子β（TGF-β）激活的lncRNA（lncRNA-ATB）被发现是肝细胞癌、胃癌和结直肠癌的预后因素。然而，关于lncRNA-ATB在肾移植中的作用知之甚少。本研究旨在评估急性肾损伤肾移植患者中lncRNA-ATB的表达，并探讨其在术后药物免疫抑制治疗中的作用。
我们检测了72例同种异体肾移植排斥反应患者和36例对照移植患者肾活检组织中lncRNA-ATB的表达。lncRNA-ATB通过慢病毒载体在肾细胞中过表达。
我们发现，与对照组相比，急性排斥反应患者中lncRNA-ATB明显上调。同时，lncRNA-ATB可影响肾细胞表型并影响免疫抑制药物的肾毒性。
总之，急性排斥反应患者中lncRNA-ATB有显著改变，可能作为急性肾排斥反应的新型生物标志物，用于识别急性排斥反应患者并预测肾功能丧失。
