Wang Shaohua, Zhang Yuyu, Yang Mingying, Ye Lupeng, Gong Lu, Qian Qiujie, Shuai Yajun, You Zhengying, Chen Yuyin, Zhong Boxiong
College of Animal Sciences, Zhejiang University, Hangzhou 310058, P.R. China.
College of Life Sciences, Zhejiang University, Hangzhou 310058, P.R. China.
PLoS One. 2016 Jul 14;11(7):e0159111. doi: 10.1371/journal.pone.0159111. eCollection 2016.
Silk fibers have many inherent properties that are suitable for their use in biomaterials. In this study, the silk fibroin was genetically modified by including a Ca-binding sequence, [(AGSGAG)6ASEYDYDDDSDDDDEWD]2 from shell nacreous matrix protein. It can be produced as fibers by transgenic silkworm. The Ca-binding activity and mineralization of the transgenic silk fibroin were examined in vitro. The results showed that this transgenic silk fibroin had relatively higher Ca-binding activity than unmodified silk fibroin. The increased Ca-binding activity could promote the usage of silk fibroin as a biomaterial in the pharmaceutical industry. This study shows the possibility of using silk fibroin as a mineralization accelerating medical material by generating genetically modified transgenic silkworm.
丝纤维具有许多适合用于生物材料的固有特性。在本研究中，通过包含来自贝壳珍珠层基质蛋白的钙结合序列[(AGSGAG)6ASEYDYDDDSDDDDEWD]2对丝素蛋白进行了基因改造。它可以由转基因蚕生产成纤维。在体外检测了转基因丝素蛋白的钙结合活性和矿化作用。结果表明，这种转基因丝素蛋白比未修饰的丝素蛋白具有相对更高的钙结合活性。钙结合活性的增加可以促进丝素蛋白在制药工业中作为生物材料的应用。这项研究表明了通过培育转基因蚕将丝素蛋白用作矿化促进医用材料的可能性。
