Institute of Infectious Diseases and Epidemiology, Tan Tock Seng Hospital, Singapore
Saw Swee Hock School of Public Health, National University of Singapore, Singapore.
J Clin Microbiol. 2017 Dec;55(12):3339-3349. doi: 10.1128/JCM.00707-17. Epub 2017 Sep 13.
Dengue detection strategies involve viral RNA, antigen, and/or antibody detection. Each strategy has its advantages and disadvantages. Optimal, user-friendly, rapid diagnostic tests based on immunochromatographic assays are pragmatic point-of-care tests (POCTs) in regions where dengue is endemic where there are limited laboratory capabilities and optimal storage conditions. Increasingly, there is a greater public health significance for a multiplexing assay that differentiates dengue from Zika or pathogens with similar clinical presentations. Although there have been many assay/platform developments toward POCTs, independent validation and implementation remain very limited. This review highlights the current key progress and challenges toward the development of a dengue POCT.
登革热检测策略包括病毒 RNA、抗原和/或抗体检测。每种策略都有其优点和缺点。在登革热流行地区，基于免疫层析检测的最佳、用户友好、快速诊断检测方法是实用的床边检测（POCT），因为这些地区的实验室能力有限，且无法满足最佳存储条件。对于能够区分登革热与寨卡病毒或具有相似临床表现的病原体的多重检测方法，其公共卫生意义越来越大。尽管已经有许多针对 POCT 的检测方法/平台的开发，但独立验证和实施仍然非常有限。本综述重点介绍了登革热 POCT 发展的当前关键进展和挑战。
