Chen Ying, Fan Huan, Meng Fanrui
Tianjin Institute of Forest and Pomology, Tianjin, China.
Tianjin Institute of Animal Husbandry and Veterinary Research, Tianjin, China.
J Sci Food Agric. 2017 Mar;97(5):1592-1598. doi: 10.1002/jsfa.7907. Epub 2016 Sep 7.
The capacity of Pleurotus ostreatus to degrade lignin was investigated in the fermentation of cornstalk. Cornstalk was incubated with P. ostreatus for 30 days, and acid-soluble and acid-insoluble lignins were assessed. The microscopic structure of cornstalk samples was studied by scanning electron microscopy (SEM), and spectroscopic characteristics were measured by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and solid state nuclear magnetic resonance ( C NMR) spectroscopy.
During fermentation of cornstalk, the proportion of acid-soluble lignin did not vary significantly (P > 0.05), but that of acid-insoluble lignin decreased gradually from 17.8% on day 0 to 7.6% on day 30 (P < 0.01). SEM revealed that the surface of cornstalk was gradually damaged with cavities increasing in number and size, forming a quasi-network structure. Crystallinity decreased from 35.0 on day 0 to 15.2 on day 30. FTIR and cross-polarization magic angle spinning (CPMAS) C NMR spectra showed that the intensity of the peaks corresponding to lignin, cellulose and hemicellulose also decreased gradually over 30 days.
Cornstalk can be effectively degraded by P. ostreatus within 30 days. Pleurotus ostreatus decreases cornstalk lignin content, potentially improving its suitability for animal feed. © 2016 Society of Chemical Industry.
在玉米秸秆发酵过程中研究了糙皮侧耳降解木质素的能力。将玉米秸秆与糙皮侧耳一起培养30天，并对酸溶性和酸不溶性木质素进行评估。通过扫描电子显微镜（SEM）研究玉米秸秆样品的微观结构，并通过X射线衍射（XRD）、傅里叶变换红外光谱（FTIR）和固态核磁共振（(^{13})C NMR）光谱测量光谱特征。
在玉米秸秆发酵过程中，酸溶性木质素的比例没有显著变化（P>0.05），但酸不溶性木质素的比例从第0天的17.8%逐渐降至第30天的7.6%（P<0.01）。扫描电子显微镜显示，玉米秸秆表面逐渐受损，空洞数量和尺寸增加，形成准网络结构。结晶度从第0天的35.0降至第30天的15.2。傅里叶变换红外光谱和交叉极化魔角旋转（CPMAS）(^{13})C NMR光谱表明，与木质素、纤维素和半纤维素相对应的峰强度在30天内也逐渐降低。
糙皮侧耳可在30天内有效降解玉米秸秆。糙皮侧耳降低了玉米秸秆的木质素含量，可能提高其对动物饲料的适用性。©2016化学工业协会。
