van Kuijk Sandra J A, Sonnenberg Anton S M, Baars Johan J P, Hendriks Wouter H, Cone John W
Animal Nutrition Group, Wageningen University, De Elst 1, 6708WD Wageningen, The Netherlands.
Plant Breeding, Wageningen University, Droevendaalsesteeg 1, 6708PB Wageningen, The Netherlands.
J Anim Sci Biotechnol. 2016 Jul 13;7:39. doi: 10.1186/s40104-016-0098-4. eCollection 2016.
The aim of this study was to optimize the fungal treatment of lignocellulosic biomass by stimulating the colonization. Wheat straw and wood chips were treated with Ceriporiopsis subvermispora and Lentinula edodes with various amounts of colonized millet grains (0.5, 1.5 or 3.0 % per g of wet weight of substrate) added to the substrates. Also, wheat straw and wood chips were chopped to either 0.5 or 2 cm. Effectiveness of the fungal treatment after 0, 2, 4, 6, or 8 wk of incubation was determined by changes in chemical composition, in vitro gas production (IVGP) as a measure for rumen degradability, and ergosterol content as a measure of fungal biomass.
Incomplete colonization was observed for C. subvermispora treated wheat straw and L. edodes treated wood chips. The different particle sizes and amounts of inoculum tested, had no significant effects on the chemical composition and the IVGP of C. subvermispora treated wood chips. Particle size did influence L. edodes treatment of wheat straw. The L. edodes treatment of 2 cm wheat straw resulted in a more selective delignification and a higher IVGP than the smaller particles. Addition of 1.5 % or 3 % L. edodes inoculum to wheat straw resulted in more selective delignification and a higher IVGP than addition of 0.5 % inoculum.
Particle size and amount of inoculum did not have an effect on C. subvermispora treatment of wood chips. At least 1.5 % L. edodes colonized millet grains should be added to 2 cm wheat straw to result in an increased IVGP and acid detergent lignin (ADL) degradation.
本研究旨在通过刺激定殖来优化木质纤维素生物质的真菌处理。用不同量（每克底物湿重0.5%、1.5%或3.0%）的定殖粟粒对小麦秸秆和木屑进行了黄孢原毛平革菌和香菇处理。此外，将小麦秸秆和木屑切碎至0.5厘米或2厘米。通过化学成分的变化、作为瘤胃降解性指标的体外产气（IVGP）以及作为真菌生物量指标的麦角固醇含量，测定了培养0、2、4、6或8周后真菌处理的有效性。
观察到黄孢原毛平革菌处理的小麦秸秆和香菇处理的木屑存在定殖不完全的情况。所测试的不同粒径和接种量对黄孢原毛平革菌处理的木屑的化学成分和IVGP没有显著影响。粒径确实影响香菇对小麦秸秆的处理。与较小颗粒相比，香菇对2厘米小麦秸秆的处理导致了更具选择性的脱木质素作用和更高的IVGP。向小麦秸秆中添加1.5%或3%的香菇接种物比添加0.5%接种物导致更具选择性的脱木质素作用和更高的IVGP。
粒径和接种量对黄孢原毛平革菌处理木屑没有影响。应向2厘米小麦秸秆中添加至少1.5%的香菇定殖粟粒，以提高IVGP和酸性洗涤木质素（ADL）的降解率。
