Zheng Chen, Cone John W, van Peer Arend, Baars Johan J P, Hendriks Wouter H
Department of Animal Science, Wageningen University & Research, Wageningen, The Netherlands.
Department of Plant Science, Wageningen University & Research, Wageningen, The Netherlands.
J Anim Physiol Anim Nutr (Berl). 2025 May;109(3):800-811. doi: 10.1111/jpn.14099. Epub 2025 Jan 10.
White rot fungi can degrade lignin and improve the nutritional value of highly lignified biomass for ruminants. We screened for excellent fungi-biomass combinations by investigating the improvement of digestibility of wheat straw, barley straw, oat straw, rapeseed straw, miscanthus, new reed, spent reed from thatched roofs, and cocoa shells after colonisation by Ceriporiopsis subvermispora (CS), Lentinula edodes (LE), and Pleurotus eryngii (PE) (indicated by increased in vitro gas production [IVGP]). First, growth was evaluated for three fungi on all types of biomass, over a period of 17 days in race tubes. CS grew faster than LE and PE on all types of biomass. LE did not grow on cocoa shells, while growth rate of CS and PE on cocoa shells was lower compared to other types of biomass. After this first screening, all types of biomass, excluding the cocoa shells, were colonised by the three fungal strains for 8 weeks. Treatment with CS and LE improved IVGP more than treatment with PE. Methane production was reduced in six combinations of biomass with CS, four with LE, and three with PE. Six types of biomass were selected for treatment with CS and four were selected for treatment with CS and LE, to determine the net improvement of nutritional value (increased IVGP corrected for dry matter loss) after 2, 4, 6, 7 and 8 weeks of treatment. The highest net improvement was found for CS and LE treated rapeseed straw (86% and 20%, respectively) and spent reed (80% and 43%, respectively). All treatments decreased dry matter, lignin and hemicellulose, the latter two both in absolute amount and content. In conclusion, net improvement of highly lignified biomasses by CS was greater than LE, with the nutritional value of rapeseed straw and spent reed being significantly improved by both fungi.
白腐真菌能够降解木质素，并提高高度木质化生物质对反刍动物的营养价值。我们通过研究被亚侧耳栓菌（CS）、香菇（LE）和平鲍菇（PE）定殖后小麦秸秆、大麦秸秆、燕麦秸秆、油菜秸秆、芒草、新芦苇、茅草屋顶的废芦苇和可可壳的消化率改善情况（以体外产气量[IVGP]增加表示），筛选出了优良的真菌-生物质组合。首先，在试管中对三种真菌在所有类型生物质上的生长情况进行了为期17天的评估。CS在所有类型生物质上的生长速度均快于LE和PE。LE在可可壳上不生长，而CS和PE在可可壳上的生长速度低于其他类型的生物质。经过首次筛选后，除可可壳外的所有类型生物质均被三种真菌菌株定殖8周。与PE处理相比，CS和LE处理对IVGP的改善更大。六种生物质与CS的组合、四种与LE的组合以及三种与PE的组合中甲烷产量均降低。选择六种类型的生物质用CS处理，四种用CS和LE处理，以确定处理2、4、6、7和8周后营养价值的净改善情况（校正干物质损失后IVGP增加）。发现CS和LE处理的油菜秸秆（分别为86%和20%）和废芦苇（分别为80%和43%）的净改善最高。所有处理均降低了干物质、木质素和半纤维素，后两者的绝对量和含量均降低。总之，CS对高度木质化生物质的净改善大于LE，两种真菌均显著提高了油菜秸秆和废芦苇的营养价值。
