Animal Nutrition Group, Wageningen University & Research, Wageningen, The Netherlands.
Plant Breeding, Wageningen University & Research, Wageningen, The Netherlands.
J Appl Microbiol. 2018 Aug;125(2):468-479. doi: 10.1111/jam.13894. Epub 2018 May 23.
In this study, the biological variation for improvement of the nutritive value of wheat straw by 12 Ceriporiopsis subvermispora, 10 Pleurotus eryngii and 10 Lentinula edodes strains was assessed. Screening of the best performing strains within each species was made based on the in vitro degradability of fungal-treated wheat straw.
Wheat straw was inoculated with each strain for 7 weeks of solid state fermentation. Weekly samples were evaluated for in vitro gas production (IVGP) in buffered rumen fluid for 72 h. Out of the 32 fungal strains studied, 17 strains showed a significantly higher (P < 0·05) IVGP compared to the control after 7 weeks (227·7 ml g OM). The three best Ceriporiopsis subvermispora strains showed a mean IVGP of 297·0 ml g OM, while the three best P. eryngii and L. edodes strains showed a mean IVGP of 257·8 and 291·5 ml g OM, respectively.
Ceriporiopsis subvermispora strains show an overall high potential to improve the ruminal degradability of wheat straw, followed by L. edodes and P. eryngii strains.
Large variation exists within and among different fungal species in the valorization of wheat straw, which offers opportunities to improve the fungal genotype by breeding.
本研究评估了 12 株 Ceriporiopsis subvermispora、10 株 Pleurotus eryngii 和 10 株 Lentinula edodes 对小麦秸秆营养价值的改善的生物学变异性。根据真菌处理过的小麦秸秆的体外降解性，对每个物种中表现最好的菌株进行了筛选。
用每个菌株对小麦秸秆进行 7 周的固态发酵接种。每周采集样品，在缓冲瘤胃液中进行 72 小时的体外产气（IVGP）评估。在所研究的 32 株真菌菌株中，有 17 株在 7 周后（227.7 ml g OM）的 IVGP 明显高于对照（P < 0.05）。三株表现最好的 Ceriporiopsis subvermispora 菌株的平均 IVGP 为 297.0 ml g OM，而三株表现最好的 P. eryngii 和 L. edodes 菌株的平均 IVGP 分别为 257.8 和 291.5 ml g OM。
Ceriporiopsis subvermispora 菌株对提高小麦秸秆的瘤胃降解性具有较高的整体潜力，其次是 L. edodes 和 P. eryngii 菌株。
不同真菌物种之间以及同一真菌物种内部存在着很大的差异，这为通过选育来提高真菌基因型提供了机会。
