Department of Isotope Biogeochemistry, Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany.
Department of Isotope Biogeochemistry, Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany.
Curr Opin Biotechnol. 2016 Oct;41:114-121. doi: 10.1016/j.copbio.2016.06.007. Epub 2016 Jul 12.
The combination of stable isotope probing (SIP), NanoSIMS imaging and microbe identification via fluorescence in situ hybridization (FISH) is often used to link identity to function at the cellular level in microbial communities. Many opportunities remain for nanoSIP to identify metabolic interactions and nutrient fluxes within syntrophic associations and obligate symbioses where exchanges can be extremely rapid. However, additional data, such as genomic potential, gene expression or other imaging modalities are often critical to deciphering the mechanisms underlying specific interactions, and researchers must keep sample preparation artefacts in mind. Here we focus on recent applications of nanoSIP, particularly where used to track exchanges of isotopically labelled molecules between organisms. We highlight metabolic interactions within syntrophic consortia, carbon/nitrogen fluxes between phototrophs and their heterotrophic partners, and symbiont-host nutrient sharing.
稳定同位素探测（SIP）、纳米二次离子质谱成像（NanoSIMS）和荧光原位杂交（FISH）微生物鉴定相结合，通常用于在微生物群落的细胞水平上连接身份和功能。纳米 SIP 有很多机会可以识别在同型共生体和专性共生体中的代谢相互作用和营养通量，在这些共生体中，交换可以非常迅速。然而，额外的数据，如基因组潜力、基因表达或其他成像方式，对于破译特定相互作用的机制通常是至关重要的，研究人员必须牢记样品制备的假象。在这里，我们重点介绍纳米 SIP 的最新应用，特别是用于跟踪同位素标记分子在生物体之间的交换。我们强调同型共生体中的代谢相互作用、光养生物与其异养伙伴之间的碳/氮通量，以及共生体-宿主的营养共享。
