Proteomics Core Facility, University of Technology Sydney, Cnr Harris and Thomas St, Ultimo, New South Wales, 2007, Australia.
The iThree Institute, University of Technology Sydney, Cnr Harris and Thomas St, Ultimo, New South Wales, 2007, Australia.
Mass Spectrom Rev. 2018 Mar;37(2):217-228. doi: 10.1002/mas.21515. Epub 2016 Jul 15.
Reproducibility has been one of the biggest hurdles faced when attempting to develop quantitative protocols for MALDI mass spectrometry. The heterogeneous nature of sample recrystallization has made automated sample acquisition somewhat "hit and miss" with manual intervention needed to ensure that all sample spots have been analyzed. In this review, we explore the last 30 years of literature and anecdotal evidence that has attempted to address and improve reproducibility in MALDI MS. Though many methods have been attempted, we have discovered a significant publication history surrounding the use of nitrocellulose as a substrate to improve homogeneity of crystal formation and therefore reproducibility. We therefore propose that this is the most promising avenue of research for developing a comprehensive and universal preparation protocol for quantitative MALDI MS analysis. © 2016 Wiley Periodicals, Inc. Mass Spec Rev 37:217-228, 2018.
重现性一直是尝试开发 MALDI 质谱定量协议时面临的最大障碍之一。由于样品重结晶的异质性，自动化样品采集有点“碰运气”，需要人工干预以确保已分析所有样品点。在这篇综述中，我们探讨了过去 30 年的文献和轶事证据，这些文献和轶事证据试图解决和提高 MALDI MS 中的重现性。尽管已经尝试了许多方法，但我们发现有关使用硝酸纤维素作为基质来改善晶体形成的均一性从而提高重现性的大量文献。因此，我们提出，这是开发用于定量 MALDI MS 分析的全面通用制备方案的最有前途的研究途径。©2016Wiley Periodicals，Inc. 质谱评论 37：217-228，2018 年。
