Dietz Donna, Schneider Roland, Papendiek Franka, Venus Joachim
Leibniz Institute for Agricultural Engineering Potsdam-Bornim, Germany.
Leibniz Institute for Agricultural Engineering Potsdam-Bornim, Germany.
J Biotechnol. 2016 Oct 20;236:26-34. doi: 10.1016/j.jbiotec.2016.07.008. Epub 2016 Jul 12.
Lactic acid is one of the most important building blocks for the production of bioplastic. Many investigations have been conducted to reduce the lactic acid production costs. In this work, the focus was put on the application of legume pressed juice or green juice as nutrient source. The pressed juice was utilized directly without prior pre-treatment and sterilization. Using two different alfalfa green juices and a clover green juice from two different harvest years as sole nutrients, non-sterile fermentations were performed at 52°C and pH 6.0 with a thermotolerant strain Bacillus coagulans AT107. The results showed that alfalfa green juices generally were more suitable for high lactic acid production than clover green juices, presumably due to the higher nitrogen content. A final titer of 98.8g/L after 30h with l(+)-lactic acid purity of >99% was obtained.
乳酸是生产生物塑料最重要的原料之一。人们已经进行了许多研究来降低乳酸的生产成本。在这项工作中，重点是将豆类压榨汁或生汁用作营养源。压榨汁未经预先处理和灭菌就直接使用。使用来自两个不同收获年份的两种不同紫花苜蓿生汁和一种三叶草生汁作为唯一营养物质，在52°C和pH 6.0条件下，用耐热菌株凝结芽孢杆菌AT107进行非无菌发酵。结果表明，紫花苜蓿生汁通常比三叶草生汁更适合高乳酸生产，这可能是由于其较高的氮含量。30小时后最终滴度达到98.8g/L，l(+)-乳酸纯度>99%。
