Department of Bioengineering, Leibniz-Institute for Agricultural Engineering and Bioeconomy, Potsdam, Germany.
Department of Bioengineering, Leibniz-Institute for Agricultural Engineering and Bioeconomy, Potsdam, Germany; Institut Químic de Sarrià (IQS), Universitat Ramon Llull, Barcelona, Spain.
Bioresour Technol. 2020 Nov;316:123949. doi: 10.1016/j.biortech.2020.123949. Epub 2020 Aug 3.
The biotechnological production of platform chemicals from renewable resources is in the scientific spotlight, as researchers seek to develop environmentally friendly and cost-efficient processes to compete with the petroleum-based ones. Lactic acid (LA) is an established platform chemical, registering an important market share, mainly owing to the increasing demand for polylactic acid. This study investigated the feasibility to produce LA from bakery waste hydrolysates and lucerne green juice (LGJ) as inexpensive substrates, using a Bacillus coagulans strain. A final LA concentration of 62.2 g/L, with a productivity of 2.59 g/(L.h) and a conversion yield of 0.57 g LA/ g bakery waste was achieved in batch fermentation mode. LA productivity reached 11.28 g/(L.h), using a continuous fermentation system coupled with cell retention membranes at a dilution rate of 0.2 h. The results indicate that bakery waste hydrolysates and LGJ can be utilized for the production of highly optical pure L(+)-LA.
从可再生资源中通过生物技术生产平台化学品是当前科学研究的热点，研究人员正在寻求开发环保且具有成本效益的工艺，以与基于石油的工艺竞争。乳酸（LA）是一种已确立的平台化学品，占据着重要的市场份额，这主要归因于对聚乳酸的需求不断增加。本研究旨在探索利用面包废物水解物和卢瑟恩绿汁（LGJ）作为廉价底物，使用凝结芽孢杆菌菌株生产 LA 的可行性。在分批发酵模式下，最终获得了 62.2 g/L 的 LA 浓度，产率为 2.59 g/（L.h），转化率为 0.57 g LA/g 面包废物。通过连续发酵系统并结合细胞保留膜在稀释率为 0.2 h 的条件下，LA 的产率达到 11.28 g/（L.h）。结果表明，面包废物水解物和 LGJ 可用于生产高光学纯 L(+)-LA。
