van Schie B J, van Dijken J P, Kuenen J G
Laboratory of Microbiology and Enzymology, Delft University of Technology, The Netherlands.
Antonie Van Leeuwenhoek. 1989;55(1):53-65. doi: 10.1007/BF02309619.
The regulation of the synthesis of the quinoprotein glucose dehydrogenase (EC 1.1.99.17) has been studied in Acinetobacter calcoaceticus LMD 79.41, an organism able to oxidize glucose to gluconic acid, but unable to grow on both compounds. Glucose dehydrogenase was synthesized constitutively in both batch and carbon-limited chemostat cultures on a variety of substrates. In acetate-limited chemostat cultures glucose dehydrogenase levels and the glucose-oxidizing capacity of whole cells were dependent on the growth rate. They strongly increased at low growth rates at which the maintenance requirement of the cells had a pronounced effect on biomass yield. Cultures grown on a mixture of acetate and glucose in carbon and energy-limited chemostat cultures oxidized glucose quantitatively to gluconic acid. However, during oxygen-limited growth on this mixture glucose was not oxidized and only very low levels of glucose dehydrogenase were detected in cell-free extracts. After introduction of excess oxygen, however, cultures or washed cell suspensions almost instantaneously gained the capacity to oxidize glucose at a high rate, by an as yet unknown mechanism.
已在醋酸钙不动杆菌LMD 79.41中研究了喹啉蛋白葡萄糖脱氢酶（EC 1.1.99.17）合成的调控，该菌能够将葡萄糖氧化为葡萄糖酸，但不能在这两种化合物上生长。在分批培养和碳限制恒化器培养中，在多种底物上，葡萄糖脱氢酶都是组成型合成的。在醋酸盐限制的恒化器培养中，葡萄糖脱氢酶水平和全细胞的葡萄糖氧化能力取决于生长速率。在低生长速率下它们会显著增加，此时细胞的维持需求对生物量产量有显著影响。在碳和能量限制的恒化器培养中，在醋酸盐和葡萄糖的混合物上生长的培养物将葡萄糖定量氧化为葡萄糖酸。然而，在该混合物上进行限氧生长期间，葡萄糖未被氧化，并且在无细胞提取物中仅检测到极低水平的葡萄糖脱氢酶。然而，引入过量氧气后，培养物或洗涤后的细胞悬液几乎立即获得了以高速率氧化葡萄糖的能力，其机制尚不清楚。
