van Schie B J, Rouwenhorst R J, van Dijken J P, Kuenen J G
Laboratory of Microbiology and Enzymology, Delft University of Technology, The Netherlands.
Antonie Van Leeuwenhoek. 1989;55(1):39-52. doi: 10.1007/BF02309618.
Glucose metabolism has been studied in two strains of Acinetobacter calcoaceticus. Strain LMD 82.3, was able to grow on glucose and possessed glucose dehydrogenase (EC 1.1.99.17). Glucose oxidation by whole cells was stimulated by PQQ, the prosthetic group of glucose dehydrogenase. PQQ not only increased the rate of glucose oxidation and gluconic acid production but also shortened the lag phase for growth on glucose. Strain LMD 79.41 also possessed glucose dehydrogenase but was unable to grow on glucose. Batch cultures and carbon-limited chemostat cultures growing on acetate in the presence of glucose oxidized the sugar to gluconic acid, which was not further metabolized. However, after prolonged cultivation on mixtures of acetate and glucose, carbon-limited chemostat cultures suddenly acquired the capacity to utilize gluconate. This phenomenon was accompanied by the appearance of gluconate kinase and a repression of isocitrate lyase synthesis. In contrast to the starter culture, cells from chemostats which had been fully adapted to gluconate utilization, were able to utilize glucose as a sole carbon and energy source in liquid and solid media.
已对两株醋酸钙不动杆菌的葡萄糖代谢进行了研究。菌株LMD 82.3能够在葡萄糖上生长，并具有葡萄糖脱氢酶（EC 1.1.99.17）。葡萄糖脱氢酶的辅基吡咯喹啉醌（PQQ）可刺激全细胞的葡萄糖氧化。PQQ不仅提高了葡萄糖氧化速率和葡萄糖酸的产量，还缩短了在葡萄糖上生长的延迟期。菌株LMD 79.41也具有葡萄糖脱氢酶，但无法在葡萄糖上生长。在葡萄糖存在的情况下，以醋酸盐为碳源进行分批培养和碳限制恒化培养时，葡萄糖被氧化为葡萄糖酸，且葡萄糖酸不再进一步代谢。然而，在醋酸盐和葡萄糖混合物上长期培养后，碳限制恒化培养物突然获得了利用葡萄糖酸的能力。这一现象伴随着葡萄糖酸激酶的出现和异柠檬酸裂解酶合成的抑制。与起始培养物不同，已完全适应利用葡萄糖酸的恒化器中的细胞能够在液体和固体培养基中利用葡萄糖作为唯一的碳源和能源。
