Belém Mônica O, de Andrade Giovana M M, Carlos Thalita M, Guazelli Carla F S, Fattori Victor, Toginho Filho Dari O, Dias Ivan F L, Verri Waldiceu A, Araújo Eduardo J A
Departamento de Histologia, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Brazil.
Departamento de Ciências Patológicas, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Brazil.
J Photochem Photobiol B. 2016 Sep;162:367-373. doi: 10.1016/j.jphotobiol.2016.07.008. Epub 2016 Jul 9.
Inflammatory bowel disease (IBD) presents intense inflammatory infiltrate, crypt abscesses, ulceration and even loss of function. Despite the clinical relevance of IBD, its current therapy remains poorly effective. Infrared wavelength phototherapy shows therapeutic potential on inflammation. Our goal was to evaluate whether light-emitting diodes (LED) at 940nm are capable of mitigating the colitis-induced inflammatory process in mice. Forty male Swiss mice were assigned into five groups: control; control treated with LED therapy; colitis without treatment; colitis treated with LED therapy; colitis treated with Prednisolone. Experimental colitis was induced by acetic acid 7.5% (pH2.5) rectal administration. LED therapy was performed with light characterized by wavelength of 940nm, 45nm bandwidth, intensity of 4.05J/cm(2), total power of 270mW and total dose of 64.8J for 4min in a single application. Colitis-induced intestinal transit delay was inhibited by LED therapy. Colitis caused an increase of colon dimensions (length, diameter, total area) and colon weight (edema), which were inhibited by LED therapy. LED therapy also decreased colitis-induced tissue gross lesion, myeloperoxidase activity, microscopic tissue damage score and the presence of inflammatory infiltrate in all intestinal layers. Furthermore, LED therapy inhibited colitis-induced IL-1β, TNF-α, and IL-6 production. We conclude LED therapy at 940nm inhibited experimental colitis-induced colon inflammation in mice, therefore, rendering it a promising therapeutic approach that deserves further investigation.
炎症性肠病（IBD）表现为强烈的炎症浸润、隐窝脓肿、溃疡甚至功能丧失。尽管IBD具有临床相关性，但其目前的治疗效果仍然不佳。红外波长光疗对炎症显示出治疗潜力。我们的目标是评估940nm的发光二极管（LED）是否能够减轻小鼠结肠炎诱导的炎症过程。40只雄性瑞士小鼠被分为五组：对照组；接受LED治疗的对照组；未治疗的结肠炎组；接受LED治疗的结肠炎组；接受泼尼松龙治疗的结肠炎组。通过直肠给予7.5%（pH2.5）的醋酸诱导实验性结肠炎。LED治疗采用波长为940nm、带宽45nm、强度4.05J/cm²、总功率270mW、总剂量64.8J、单次照射4分钟的光进行。LED治疗抑制了结肠炎诱导的肠道转运延迟。结肠炎导致结肠尺寸（长度、直径、总面积）和结肠重量（水肿）增加，而LED治疗抑制了这些增加。LED治疗还减少了结肠炎诱导的组织大体病变、髓过氧化物酶活性、微观组织损伤评分以及所有肠层中炎症浸润的存在。此外，LED治疗抑制了结肠炎诱导的IL-1β、TNF-α和IL-6的产生。我们得出结论，940nm的LED治疗抑制了小鼠实验性结肠炎诱导的结肠炎症，因此，使其成为一种有前途的治疗方法，值得进一步研究。
