Sakar Ebru, Unver Hulya, Ercisli Sezai
Department of Horticulture, Agricultural Faculty, Harran University, Sanliurfa, Turkey.
Faculty of Agriculture and Natural Science, Duzce University, Duzce, Turkey.
Biochem Genet. 2016 Dec;54(6):842-853. doi: 10.1007/s10528-016-9761-x. Epub 2016 Jul 16.
Olive (Olea europaea) is an ancient and important crop in both olive oil production and table use. It is important to identify the genetic diversity of olive genetic resources for cultivar development and evaluation of olive germplasm. In the study, 14 microsatellite markers (UDO4, UDO8, UDO9, UDO11, UDO12, UDO22, UDO24, UDO26, UDO28, DCA9, DCA11, DCA13, DCA15, and DCA18) were used to assess the genetic variation on 76 olive (Olea europaea L.) genotypes from Mardin province together with 6 well-known Turkish and 4 well-known foreign reference cultivars. All microsatellite markers showed polymorphism and the number of alleles varied between 9 and 22, with an average of 14.57. The most informative loci were DCA 11 (22 alleles) and DCA 9 (21 alleles). Dendrogram based on genetic distances was constructed for the 86 olive genotypes/cultivars, which revealed the existence of different clusters. The high genetic similarity was evident between Bakırkire2 and Zinnar5 (0.74) genotypes, while the most genetically divergent genotypes were Gürmeşe5 and Yedikardeşler2 (0.19). It was concluded that there was abundant SSR polymorphism in olive germplasm in southern Anatolia in Turkey and could be important for future breeding activities.
油橄榄（Olea europaea）在橄榄油生产和食用方面都是一种古老且重要的作物。识别油橄榄遗传资源的遗传多样性对于品种培育和油橄榄种质评估至关重要。在本研究中，使用了14个微卫星标记（UDO4、UDO8、UDO9、UDO11、UDO12、UDO22、UDO24、UDO26、UDO28、DCA9、DCA11、DCA13、DCA15和DCA18）来评估来自马尔丁省的76个油橄榄（Olea europaea L.）基因型以及6个著名的土耳其参考品种和4个著名的国外参考品种的遗传变异。所有微卫星标记均表现出多态性，等位基因数量在9至22之间变化，平均为14.57。信息含量最高的位点是DCA 11（22个等位基因）和DCA 9（21个等位基因）。基于遗传距离构建了86个油橄榄基因型/品种的树状图，结果显示存在不同的聚类。Bakırkire2和Zinnar5基因型之间的遗传相似性很高（0.74），而遗传差异最大的基因型是Gürmeşe5和Yedikardeşler2（0.19）。研究得出结论，土耳其安纳托利亚南部的油橄榄种质中存在丰富的SSR多态性，这对未来的育种活动可能具有重要意义。
