基于 SSR 标记的橄榄（Olea europaea）DNA 指纹图谱和品种鉴定。

SSR marker-based DNA fingerprinting and cultivar identification of olives (Olea europaea).

机构信息

Department of Horticulture, Faculty of Agriculture, Ataturk University, 25240 Erzurum, Turkey.

出版信息

Biochem Genet. 2011 Oct;49(9-10):555-61. doi: 10.1007/s10528-011-9430-z. Epub 2011 Apr 8.

Abstract

Four well-known commercial olive cultivars (Domat, Edremit, Gemlik, and Memecik) and six local cultivars (Ziraat, Isrange, Tuz, Patos, Yag, and Marantelli) from northeastern Turkey were analyzed for genetic diversity and relationships using seven SSR primers (DCA-4, DCA-09, DCA-11, DCA-16, DCA-17, GAPU-89, UDO-14). The number of markers ranged from 3 (DCA-04 and DCA-17) to 6 (DCA-11, DCA-16, GAPU-89), with an average of 4.57 alleles per primer. UPGMA cluster analysis based on a simple matching similarity matrix grouped cultivars into two main clusters. Three pairs of cultivars (Ziraat and Gemlik, Isrange and Tuz, and Patos and Yag) were thought to be different cultivars although they produced identical SSR profiles. The results indicate the efficiency of SSR markers for evaluation of genetic diversity in olives and identification of misnamed individuals of the same genotype.

摘要

对来自土耳其东北部的四个著名商业橄榄品种（Domat、Edremit、Gemlik 和 Memecik）和六个本地品种（Ziraat、Isrange、Tuz、Patos、Yag 和 Marantelli）进行了遗传多样性和关系分析，使用了 7 个 SSR 引物（DCA-4、DCA-09、DCA-11、DCA-16、DCA-17、GAPU-89、UDO-14）。标记的数量从 3 个（DCA-04 和 DCA-17）到 6 个（DCA-11、DCA-16、GAPU-89）不等，每个引物的平均等位基因数为 4.57。基于简单匹配相似性矩阵的 UPGMA 聚类分析将品种分为两个主要聚类。虽然三个品种组合（Ziraat 和 Gemlik、Isrange 和 Tuz、以及 Patos 和 Yag）产生了相同的 SSR 图谱，但被认为是不同的品种。结果表明 SSR 标记在评估橄榄遗传多样性和鉴定相同基因型的命名错误个体方面具有效率。