Slama-Schwok A, Rougée M, Ibanez V, Geacintov N E, Montenay-Garestier T, Lehn J M, Hélène C
Laboratoire de Biophysique, Muséum National d'Histoire Naturelle, Paris, France.
Biochemistry. 1989 Apr 18;28(8):3234-42. doi: 10.1021/bi00434a018.
The interactions of dimethyldiazaperopyrenium dication (1) with DNA have been studied by spectroscopic methods: absorption, static and dynamic fluorescence, and linear dichroism. 1 binds strongly to DNA at 250 mM NaCl, with a higher affinity for G-C pairs as compared to A-T pairs. The dye fluorescence is enhanced when it is bound to A-T pairs, whereas the emission is quenched in the vicinity of G-C pairs. Evidence for intercalation has been obtained via energy transfer and linear dichroism measurements.
已通过光谱方法（吸收光谱、静态和动态荧光光谱以及线性二色性）研究了二甲基二氮杂芘二价阳离子（1）与DNA的相互作用。在250 mM NaCl条件下，1与DNA强烈结合，与A - T碱基对相比，对G - C碱基对具有更高的亲和力。当该染料与A - T碱基对结合时，其荧光增强，而在G - C碱基对附近发射则被淬灭。已通过能量转移和线性二色性测量获得了嵌入作用的证据。
