Fung L W, Narasimhan C, Lu H Z, Westerman M P
Department of Chemistry, Loyola University of Chicago IL 60626.
Biochim Biophys Acta. 1989 Jun 26;982(1):167-72. doi: 10.1016/0005-2736(89)90188-0.
We have measured the diffusional water permeability of sickle cell anemia red blood cells under isotonic conditions using pulsed nuclear magnetic resonance (NMR) techniques. We have found that the equilibrium diffusional permeability for sickle cells is about 1.61.10(-3) cm/s, or about 60% of the value measured for normal cells. This abnormality is not related to the heterogeneity generally found in cell populations in sickle red cells with different mean corpuscular hemoglobin concentrations. We speculate that the abnormality of water exchange under isotonic conditions in sickle cells reflects an alteration of membrane proteins responsible for water exchange, possibly caused by oxidation of Band 3 proteins.
我们使用脉冲核磁共振（NMR）技术测量了等渗条件下镰状细胞贫血红细胞的扩散水渗透性。我们发现镰状细胞的平衡扩散渗透率约为1.6×10⁻³厘米/秒，约为正常细胞测量值的60%。这种异常与镰状红细胞中不同平均红细胞血红蛋白浓度的细胞群体中普遍存在的异质性无关。我们推测，镰状细胞在等渗条件下的水交换异常反映了负责水交换的膜蛋白的改变，可能是由带3蛋白的氧化引起的。
