McCoy T J, Grasel T G, Okkema A Z, Cooper S L
Department of Chemical Engineering, University of Wisconsin, Madison 53706.
Biomaterials. 1989 May;10(4):243-50. doi: 10.1016/0142-9612(89)90100-2.
Thrombus deposition was measured on NHLBI-DTB Primary Reference Material polyethylene (PRM-PE) and polydimethylsiloxane (PRM-SR) and their commercially available counterparts, surgical grade Intramedic polyethylene and Dow Corning Silastic. Canine blood-contacting experiments evaluating short-term (up to 60 min) and longer-term (up to 24 h) thrombus deposition were used to quantitate adherent platelets on the lumenal surface of test materials ex vivo. A similar pattern of thrombus deposition and detachment was observed for all materials in both acute and chronic blood contact. Although differences in the wall shear rates affected the absolute numbers of adherent platelets, the relative levels of thrombus deposition showed similarities between the two experiments, with the polyethylene materials as a group showing slightly less deposition than the silicone rubber materials. The PRM-PE showed the least thrombus deposition at extended exposure to blood. The PRM-SR showed the most thrombus deposition in the acute term. The overall similarity in blood compatibility and surface properties indicates the need for the inclusion of less thromboresistant and more polar reference materials.
在国立心、肺、血液研究所 - 双腔管主要参考材料聚乙烯（PRM - PE）和聚二甲基硅氧烷（PRM - SR）及其市售同类产品，即外科级医用聚乙烯和道康宁硅橡胶上测量血栓沉积。采用犬类血液接触实验评估短期（长达60分钟）和长期（长达24小时）血栓沉积，以在体外定量测试材料管腔表面上附着的血小板。在急性和慢性血液接触中，所有材料均观察到相似的血栓沉积和脱落模式。尽管壁面剪切速率的差异影响了附着血小板的绝对数量，但血栓沉积的相对水平在两个实验之间显示出相似性，聚乙烯材料组的沉积略少于硅橡胶材料。在长时间接触血液时，PRM - PE显示出最少的血栓沉积。PRM - SR在急性期显示出最多的血栓沉积。血液相容性和表面特性的总体相似性表明需要纳入血栓抗性较低且极性更强的参考材料。
