Spilizewski K L, Marchant R E, Anderson J M, Hiltner A
Biomaterials. 1987 Jan;8(1):12-7. doi: 10.1016/0142-9612(87)90021-4.
In vivo leucocyte interactions with the NHLBI-DTB primary reference materials, low density polyethylene (LDPE) and silica-free polydimethylsiloxane (PDMS), were qualitatively and quantitatively characterized using a cage implant system over a 21 d implantation period. Scanning electron microscopy (SEM) and cytochemical staining procedures were utilized to observe the cellular events occurring at the leucocyte/biomaterial interface. The results showed that more cells adhered to the PDMS surface than the LDPE surface at days 4 and 7. The differential analysis revealed that mononuclear cells, presumably macrophages, preferentially adhered to both polymer surfaces. By day 21, there were more very large (greater than 20 nuclei per cell) foreign body giant cells (FBGCs) present on the PDMS surface than the LDPE surface. The phagocytic capabilities of the adhered cells, including the FBGCs, decreased to a greater extent on the PDMS surface, corresponding to the earlier and more extensive spreading of these cells observed in the morphological analysis.
在一个21天的植入期内，使用笼式植入系统对体内白细胞与美国国立心肺血液研究所 - 药物开发和治疗学部（NHLBI - DTB）的主要参考材料（低密度聚乙烯（LDPE）和无二氧化硅聚二甲基硅氧烷（PDMS））之间的相互作用进行了定性和定量表征。利用扫描电子显微镜（SEM）和细胞化学染色程序来观察在白细胞/生物材料界面发生的细胞事件。结果显示，在第4天和第7天，附着在PDMS表面的细胞比LDPE表面的更多。差异分析表明，单核细胞，可能是巨噬细胞，优先附着在两种聚合物表面。到第21天，PDMS表面存在的非常大的（每个细胞大于20个细胞核）异物巨细胞（FBGCs）比LDPE表面更多。在PDMS表面，包括FBGCs在内的附着细胞的吞噬能力下降幅度更大，这与形态学分析中观察到的这些细胞更早、更广泛的铺展相对应。
