Goto Hiroko, Qadis Abdul Qadir, Kim Yo-Han, Ikuta Kentaro, Ichijo Toshihiro, Sato Shigeru
Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka, Iwate 020-8550, Japan.
J Vet Med Sci. 2016 Nov 1;78(10):1595-1600. doi: 10.1292/jvms.16-0211. Epub 2016 Jul 14.
Effects of a bacterial probiotic (BP) on ruminal fermentation and plasma metabolites were evaluated in four Holstein cattle (body weight, 645 ± 62 kg; mean ± SD) with induced subacute ruminal acidosis (SARA). SARA was induced by feeding a SARA-inducing diet, and thereafter, 20, 50 or 100 g per head of a commercial BP was administered for 7 consecutive days during the morning feeding. Cattle without BP served as the control. The 24-hr mean ruminal pH in the control was lower, whereas those in the BP groups administered 20 or 50 g were significantly higher compared to the control from days 2 to 7. Circadian patterns of the 1-hr mean ruminal pH were identical (6.4-6.8) among all cattle receiving BP. Although the mean minimum pH in the control on day -7 and day 0 was <5.8, the pH in the treatment groups on day 7 was >5.8 and significantly higher than that of the control group ( >5.2). Ruminal volatile fatty acid (VFA) concentrations were not affected by BP treatment; however, the BP groups had lower lactic acid levels compared with the control group at 20:00 on day 7. Additionally, non-esterified fatty acid levels decreased from 8:00 to 20:00 in all BP groups on day 7. These results suggest that administration of 20 to 50 g of a multi-strain BP for 7 days might improve the low pH and high lactic acid level of the ruminal fluid in SARA cattle.
在四头荷斯坦奶牛（体重645±62千克；均值±标准差）中评估了一种细菌益生菌（BP）对瘤胃发酵和血浆代谢物的影响，这些奶牛被诱导患有亚急性瘤胃酸中毒（SARA）。通过饲喂诱导SARA的日粮来诱导SARA，此后，在早晨饲喂期间连续7天每天每头奶牛给予20、50或100克商业BP。未给予BP的奶牛作为对照。对照组的24小时平均瘤胃pH较低，而在第2至7天，给予20克或50克BP的组的瘤胃pH显著高于对照组。所有接受BP的奶牛1小时平均瘤胃pH的昼夜模式相同（6.4 - 6.8）。尽管在第 -7天和第0天对照组的平均最低pH<5.8，但在第7天治疗组的pH>5.8且显著高于对照组（>5.2）。瘤胃挥发性脂肪酸（VFA）浓度不受BP处理的影响；然而，在第7天20:00时，BP组的乳酸水平低于对照组。此外，在第7天所有BP组中，非酯化脂肪酸水平从8:00到20:00均有所下降。这些结果表明，连续7天给予20至50克多菌株BP可能会改善患有SARA的奶牛瘤胃液的低pH和高乳酸水平。
