Xu Weihong, Xu Bin, Yao Yiting, Yu Xiaoling, Cao Hua, Zhang Jun, Liu Jie, Sheng Huiming
Department of Clinical Laboratory of Shanghai Tongren Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, P.R. China.
Institute of Digestive Disease of Huashan Hospital, Fudan University, Shanghai, P.R. China.
Oncol Rep. 2016 Sep;36(3):1411-8. doi: 10.3892/or.2016.4941. Epub 2016 Jul 15.
Colorectal cancer (CRC) is one of the most common malignancies worldwide. Tripartite motif-containing 29 (TRIM29) is a member of TRIM proteins family, which plays diverse physiological and pathological roles in humans. Recent studies found that TRIM29 expressed highly in CRC and promoted cell growth in vitro. However, its function in the metastasis of CRC has not been studied. In the present study, we confirmed the previous report that TRIM29 was upregulated in CRC tissues and high levels of TRIM29 expression were associated with poor overall survival of patients. Moreover, TRIM29 knockdown significantly reduced cancer cell proliferation via notably inducing cell cycle arrest and cell apoptosis. Silencing of TRIM29 significantly inhibited the migration and invasion ability of CRC cells. The protein levels of apoptosis‑, migration‑ and invasion‑related proteins were also changed after TRIM29 knockdown. Furthermore, phosphorylation levels of JAK2 and STAT3 were clearly reduced in TRIM29 knockdown cells, indicating a possible mechanism underlying its effects on colorectal carcinogenesis. Collectively, TRIM29 may exert oncogenic effects in CRC cells via regulating JAK2/STAT3 signaling.
结直肠癌（CRC）是全球最常见的恶性肿瘤之一。含三联基序蛋白29（TRIM29）是TRIM蛋白家族的成员之一，在人类中发挥着多种生理和病理作用。最近的研究发现，TRIM29在结直肠癌中高表达，并在体外促进细胞生长。然而，其在结直肠癌转移中的作用尚未得到研究。在本研究中，我们证实了之前的报道，即TRIM29在结直肠癌组织中上调，且TRIM29的高表达水平与患者的总体生存率低相关。此外，敲低TRIM29通过显著诱导细胞周期停滞和细胞凋亡，显著降低癌细胞增殖。沉默TRIM29显著抑制结直肠癌细胞的迁移和侵袭能力。敲低TRIM29后，凋亡、迁移和侵袭相关蛋白的水平也发生了变化。此外，在敲低TRIM29的细胞中，JAK2和STAT3的磷酸化水平明显降低，这表明其对结直肠癌发生作用的潜在机制。总的来说，TRIM29可能通过调节JAK2/STAT3信号通路在结直肠癌细胞中发挥致癌作用。
