Department of General Surgery, Shanghai Jiao Tong University Affiliated First People's Hospital, 85 Wujin Road, Shanghai, 200080, People's Republic of China.
Med Oncol. 2013 Dec;30(4):715. doi: 10.1007/s12032-013-0715-4. Epub 2013 Sep 28.
Tripartite motif-containing 29 (TRIM29), also known as ataxia-telangiectasia group D, is structurally a member of the tripartite motif family of proteins, which characterized by the conserved RING finger, B-box, and coiled-coil domains. TRIM29 functions as an oncogene or a tumor suppressor depending on the tumor types. In this study, we aim to evaluate whether TRIM29 affects the tumorigenesis and progression of colorectal cancer. The expression of TRIM29 was investigated using real-time PCR in 40 pairs of colorectal cancer tissues and immunohistochemistry on a tissue microarray containing 203 cases of primary colorectal cancer paired with non-cancerous tissues. Down-regulation of TRIM29 was achieved by transient transfection in RKO cell lines, and the effects of TRIM29 on tumor proliferation were evaluated by MTT and plate colony formation assays. Results indicated that TRIM29 expression was much higher in colorectal cancer tissues and significantly associated with the depth of tumor invasion, lymph node metastasis, distant metastasis, histological differentiation, vascular invasion, ki-67 index, and advanced tumor stage. Patients with TRIM29-positive tumors had a higher recurrence rate and poorer survival than patients with TRIM29-negative tumors. In multivariate analyses, the TRIM29 expression was an independent factor for determining colorectal cancer prognosis after surgery. Moreover, down-regulation of TRIM29 inhibited tumor cell proliferation in vitro. In conclusion, TRIM29 plays an important role in promoting colorectal cancer progression. Our findings suggest that TRIM29 may serve as a novel biomarker for tumor recurrence and survival for colorectal cancer patients.
三结构域蛋白 29（TRIM29），也称为共济失调毛细血管扩张症突变基因 D，在结构上是三结构域蛋白家族的成员，其特征是保守的 RING 指、B 盒和卷曲螺旋结构域。TRIM29 作为癌基因或肿瘤抑制因子的功能取决于肿瘤类型。在本研究中，我们旨在评估 TRIM29 是否影响结直肠癌的发生和发展。使用实时 PCR 检测 40 对结直肠癌组织中 TRIM29 的表达，并使用包含 203 例原发性结直肠癌及其配对非癌组织的组织微阵列进行免疫组织化学检测。通过瞬时转染在 RKO 细胞系中下调 TRIM29 的表达，并通过 MTT 和平板集落形成测定评估 TRIM29 对肿瘤增殖的影响。结果表明，TRIM29 在结直肠癌组织中的表达明显升高，与肿瘤浸润深度、淋巴结转移、远处转移、组织学分化、血管侵犯、ki-67 指数和晚期肿瘤分期显著相关。TRIM29 阳性肿瘤患者的复发率和生存率均低于 TRIM29 阴性肿瘤患者。多因素分析显示，TRIM29 表达是手术后结直肠癌预后的独立因素。此外，下调 TRIM29 抑制了肿瘤细胞在体外的增殖。总之，TRIM29 在促进结直肠癌进展中发挥重要作用。我们的研究结果表明，TRIM29 可能作为结直肠癌患者肿瘤复发和生存的新的生物标志物。
