多棘海盘车来源的鞘氨醇碱对培养的正常人表皮角质形成细胞中神经酰胺从头合成的影响。

Effects of Asterias amurensis-derived Sphingoid Bases on the de novo Ceramide Synthesis in Cultured Normal Human Epidermal Keratinocytes.

作者信息

Mikami Daisuke, Sakai Shota, Sasaki Shigefumi, Igarashi Yasuyuki

机构信息

Laboratory of Biomembrane and Biofunctional Chemistry, Graduate School of Advanced Life Science, and Frontier Research Center for Post-Genome Science and Technology, Hokkaido University.

出版信息

J Oleo Sci. 2016 Aug 1;65(8):671-80. doi: 10.5650/jos.ess16051. Epub 2016 Jul 15.

DOI:10.5650/jos.ess16051

PMID:27430385

Abstract

Asterias amurensis starfish provide several bioactive species in addition to being fishery waste. Glucosyl ceramides (GlcCers) were extracted from the viscera of these starfish and were isolated by silica gel column chromatography. Degraded GlcCers generated A. amurensis sphingoid bases (ASBs) that mainly consisted of the triene-type bases d18:3 and 9-methyl-d18:3. The effect of these bases on ceramide synthesis and content were analyzed using normal human epidermal keratinocytes (NHEKs). The bases significantly enhanced the de novo ceramide synthesis and gene expression in NHEKs for proteins, such as serine-palmitoyltransferase and ceramide synthase. Total ceramide, GlcCer, and sphingomyelin contents increased dramatically upon ASB treatment. In particular, GlcCer bearing very-long-chain fatty acids (≥C28) exhibited a significant content increase. These ASB-induced enhancements on de novo ceramide synthesis were only observed in undifferentiated NHEKs. This stimulation of the de novo sphingolipid synthesis may improve skin barrier functions.

摘要

除了作为渔业废弃物外，多棘海盘车海星还能提供多种生物活性物质。从这些海星的内脏中提取出了糖基神经酰胺（GlcCers），并通过硅胶柱色谱法进行分离。降解后的GlcCers产生了多棘海盘车鞘氨醇碱（ASBs），其主要由三烯型碱d18:3和9-甲基-d18:3组成。使用正常人表皮角质形成细胞（NHEKs）分析了这些碱对神经酰胺合成和含量的影响。这些碱显著增强了NHEKs中从头神经酰胺合成以及丝氨酸-棕榈酰转移酶和神经酰胺合酶等蛋白质的基因表达。经ASB处理后，总神经酰胺、GlcCer和鞘磷脂含量显著增加。特别是，含有极长链脂肪酸（≥C28）的GlcCer含量显著增加。这些ASB诱导的从头神经酰胺合成增强仅在未分化的NHEKs中观察到。这种对从头鞘脂合成的刺激可能会改善皮肤屏障功能。