Chen Wen-Ming, Liu Li-Ping, Chen Chaolun Allen, Wang Jih-Terng, Sheu Shih-Yi
Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Research Center for Biodiversity, Academia Sinica, Nangang 115, Taipei, Taiwan, ROC.
Int J Syst Evol Microbiol. 2016 Oct;66(10):4077-4084. doi: 10.1099/ijsem.0.001313. Epub 2016 Jul 15.
A bacterial strain, designated CL-22T, was isolated from an encrusting pore coral, Montipora aequituberculata, collected off the coast of Southern Taiwan. Its taxonomic position was investigated using a polyphasic approach. Cells of strain CL-22T were Gram-stain-negative, aerobic, motile by means of a single polar flagellum, rod-shaped and formed yellow colonies. Optimal growth occurred at 30 °C, pH 6.5-7 and in 2 % (w/v) NaCl. A neighbour-joining phylogenetic tree, based on 16S rRNA gene sequences, showed that strain CL-22T fell into the clade comprising the type strains of species of the genus Thalassotalea. Strain CL-22T exhibited 16S rRNA gene sequence similarity values of 94.7-97.1 % to the type strains of species of the genus Thalassotalea. The major fatty acids (>10 %) of strain CL-22T were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0. The predominant isoprenoid quinone was Q-8. The major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The genomic DNA G+C content of strain CL-22T was 41.2 mol%. The differential phenotypic properties, together with the phylogenetic inference, demonstrate that strain CL-22T should be classified as a novel species of the genus Thalassotalea; the name Thalassotalea montiporae sp. nov. is proposed. The type strain is CL-22T (=LMG 24827T=BCRC 17940T).
从台湾南部海岸采集的包被孔珊瑚平瘤滨珊瑚（Montipora aequituberculata）中分离出一株细菌，命名为CL-22T。采用多相分类法研究其分类地位。CL-22T菌株细胞革兰氏染色阴性，好氧，具单根极生鞭毛，呈杆状，形成黄色菌落。最适生长温度为30 °C，pH值为6.5 - 7，NaCl浓度为2 %（w/v）。基于16S rRNA基因序列构建的邻接法系统发育树表明，CL-22T菌株属于包含海栖嗜盐菌属（Thalassotalea）各物种模式菌株的进化枝。CL-22T菌株与海栖嗜盐菌属各物种模式菌株的16S rRNA基因序列相似性值为94.7 - 97.1 %。CL-22T菌株的主要脂肪酸（>10 %）为特征性总和3（C16 : 1ω7c和/或C16 : 1ω6c）和C16 : 0。主要类异戊二烯醌为Q-8。主要极性脂为磷脂酰乙醇胺和磷脂酰甘油。CL-22T菌株的基因组DNA G+C含量为41.2 mol%。其不同的表型特征以及系统发育推断表明，CL-22T菌株应被归类为海栖嗜盐菌属的一个新物种；提议将其命名为平瘤滨珊瑚海栖嗜盐菌（Thalassotalea montiporae sp. nov.）。模式菌株为CL-22T（=LMG 24827T=BCRC 17940T）。
