Sheu Shih-Yi, Xie Pei-Bei, Sheu Der-Shyan, Tang Sen-Lin, Chen Wen-Ming
Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Biodiversity Research Center, Academia Sinica, Taipei, Taiwan, ROC.
Int J Syst Evol Microbiol. 2018 Jan;68(1):432-437. doi: 10.1099/ijsem.0.002529. Epub 2017 Dec 13.
Strain Eup a-2, isolated from the torch coral Euphyllia glabrescens, was characterized using a polyphasic taxonomy approach. Cells of strain Eup a-2 were Gram-negative, aerobic and motile by three polar flagella and formed translucent colonies. Optimal growth occurred at 25 °C, pH 6-8 and in the presence of 2-4 % NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Eup a-2 belonged to the genus Litoribrevibacter and showed the highest levels of sequence similarity with respect to Litoribrevibacter albus Y32 (97.8 %). Strain Eup a-2 contained summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0 as the predominant fatty acids. The predominant isoprenoid quinone was Q-8. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphophatidylglycerol. Genomic DNA G+C content of strain Eup a-2 was 49.1 mol%. The DNA-DNA hybridization value for strain Eup a-2 with L. albus Y32 was less than 30 %. Differential phenotypic properties, together with the phylogenetic inference, demonstrate that strain Eup a-2 should be classified as a novel species of the genus Litoribrevibacter, for which the name Litoribrevibactereuphylliae sp. nov. is presented. The type strain is Eup a-2 (=BCRC 81004=LMG 29725=KCTC 52438).
从火炬珊瑚（Euphyllia glabrescens）中分离出的菌株Eup a-2，采用多相分类法进行了表征。菌株Eup a-2的细胞为革兰氏阴性、需氧，通过三根极生鞭毛运动，并形成半透明菌落。在25°C、pH 6 - 8以及2 - 4% NaCl存在的条件下生长最佳。基于16S rRNA基因序列的系统发育分析表明，菌株Eup a-2属于Litoribrevibacter属，与白色Litoribrevibacter albus Y32的序列相似性最高（97.8%）。菌株Eup a-2以特征性总和3（C16:1ω7c和/或C16:1ω6c）和C16:0作为主要脂肪酸。主要的类异戊二烯醌为Q-8。主要的极性脂为磷脂酰乙醇胺、磷脂酰甘油和双磷脂酰甘油。菌株Eup a-2的基因组DNA G + C含量为49.1 mol%。菌株Eup a-2与白色Litoribrevibacter albus Y32的DNA - DNA杂交值小于30%。不同的表型特征以及系统发育推断表明，菌株Eup a-2应被归类为Litoribrevibacter属的一个新物种，为此提出名为Litoribrevibacter euphylliae sp. nov.。模式菌株为Eup a-2（=BCRC 81004 = LMG 29725 = KCTC 52438）。
