Calik Ali, Ceylan Ahmet, Ekim Burcu, Adabi Shahram Golzar, Dilber Furkan, Bayraktaroglu Alev Gurol, Tekinay Turgay, Özen Doğukan, Sacakli Pinar
Department of Animal Nutrition & Nutritional Diseases, Faculty of Veterinary Medicine, Ankara University, Ankara, 06110, Turkey.
Department of Histology Embryology, Faculty of Veterinary Medicine, Ankara University, Ankara, 06110, Turkey.
Poult Sci. 2017 Jan 1;96(1):169-183. doi: 10.3382/ps/pew218. Epub 2016 Jul 18.
This study evaluated the effect of intra-amniotic synbiotic inclusion and continued synbiotic supplementation in the diet on the performance, intestinal epithelium integrity, and cecal microflora of broiler chickens. In Experiment 1, 510 eggs containing viable embryos were divided into 3 groups of 170 eggs each. The first group was not injected and served as a negative control ( NC: ). The next group was injected with 0.9% NaCl and was the positive control ( PC: ). The synbiotic-injected group ( S: ) was injected with a 0.5% inulin and 1 × 10 Enterococcus faecium solution. The non-injected and synbiotic injected groups were further divided into 2 sets for Experiment 2 and the birds were offered either a basal or synbiotic supplemented diet (1% inulin and 2 × 10 E. faecium cfu/kg feed). One hundred ninety-six broiler hatchlings were randomly allocated in a 2 × 2 factorial arrangement that included an intra-amniotic treatment (non-injected or synbiotic injected) and a dietary treatment (basal or synbiotic supplemented diet). The results showed that the administration of an intra-amniotic synbiotic to embryonated eggs on d 17 of incubation did not affect the hatchability or hatching weight of the birds. However, intra-amniotic synbiotic inclusion had a positive effect on FCR at d 0 to 42 (P = 0.041) and d 22 to 42 (P = 0.036). There was no significant interaction effect on the growth performance of the birds between the intra-amniotic and dietary synbiotic treatment during different or entire experimental periods. Villus height and goblet and proliferating cell nuclear antigen ( PCNA: ) positive cell counts were positively influenced by intra-amniotic and dietary synbiotic treatments. Our results also indicated that intra-amniotic synbiotic injection followed by dietary supplementation with a synbiotic significantly increased Lactobacillus colonization and decreased coliform population in the broiler cecum. Cecal butyric acid concentration increased proportionally to the cecal Lactobacillus count with dietary synbiotic supplementation on d 42. In summary, combined intra-amniotic and dietary synbiotic treatments improved broiler intestinal integrity and increased cecal beneficial bacteria populations.
本研究评估了羊膜内添加合生元以及在日粮中持续补充合生元对肉鸡生产性能、肠道上皮完整性和盲肠微生物区系的影响。在实验1中，将510枚含有活胚的鸡蛋分为3组，每组170枚。第一组不注射，作为阴性对照（NC）。第二组注射0.9%氯化钠溶液，作为阳性对照（PC）。合生元注射组（S）注射0.5%菊粉和1×10⁹屎肠球菌溶液。未注射组和合生元注射组进一步分为2组用于实验2，给雏鸡提供基础日粮或添加合生元的日粮（1%菊粉和2×10⁹屎肠球菌cfu/kg饲料）。196只肉雏鸡按2×2析因设计随机分配，包括羊膜内处理（未注射或注射合生元）和日粮处理（基础日粮或添加合生元的日粮）。结果表明，在孵化第17天给胚胎蛋羊膜内注射合生元不影响雏鸡的孵化率或出雏重。然而，羊膜内添加合生元对0至42日龄（P = 0.041）和22至42日龄（P = 0.036）的料重比有积极影响。在不同或整个实验期内，羊膜内和日粮合生元处理对雏鸡生长性能没有显著的交互作用。绒毛高度、杯状细胞和增殖细胞核抗原（PCNA）阳性细胞计数受到羊膜内和日粮合生元处理的积极影响。我们的结果还表明，羊膜内注射合生元后再在日粮中添加合生元显著增加了肉鸡盲肠中乳酸杆菌的定植并减少了大肠菌群数量。在42日龄时，日粮添加合生元后，盲肠丁酸浓度与盲肠乳酸杆菌数量成比例增加。总之，羊膜内和日粮合生元联合处理改善了肉鸡肠道完整性并增加了盲肠有益菌数量。
