一种用于肺吸虫病大规模筛查的工具：一种针对尿液样本的酶联免疫吸附测定法。
A tool for mass-screening of paragonimiasis: an enzyme-linked immunosorbent assay with urine samples.
作者信息
Qiu Xu Guang, Nakamura-Uchiyama Fukumi, Nawa Yukifumi, Itoh Makoto
机构信息
Department of Microbiology and Immunology, Aichi Medical University School of Medicine, Nagakute, Aichi 480-1195 Japan.
Department of Pathogen, Infection and Immunity, Nara Medical University, Kashihara, Nara 634-8521 Japan.
出版信息
Trop Med Health. 2016 Jun 29;44:19. doi: 10.1186/s41182-016-0019-4. eCollection 2016.
BACKGROUND
Paragonimiasis is one of the foodborn trematodiases and number of the patients was estimated to be about 23 million around the world. To obtain good compliance of people for the surveillance of paragonimiasis, an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of paragonimiasis with unconcentrated urine samples was developed.
RESULTS
Paragonimus westermani antigen specific IgG and IgG4 were detected in urine samples from paragonimiasis patients and the levels correlated well with those detected in the paired serum samples. Cross-reactions observed among other trematodiasis and a tuberculosis patient with the antigen specific IgG were much reduced by detecting the antigen specific IgG4; 9.2 % to 2.3 %.
CONCLUSIONS
The ELISA with urine samples, which are collected safely and easily, will be a useful tool for a mass-screening of paragonimiasis.
背景
肺吸虫病是食源性吸虫病之一，据估计全球患者人数约为2300万。为提高人们对肺吸虫病监测的依从性，开发了一种用于诊断肺吸虫病的酶联免疫吸附测定（ELISA），该方法使用未浓缩的尿液样本。
结果
在肺吸虫病患者的尿液样本中检测到卫氏并殖吸虫抗原特异性IgG和IgG4，其水平与配对血清样本中检测到的水平高度相关。通过检测抗原特异性IgG4，其他吸虫病患者和一名结核病患者与抗原特异性IgG之间的交叉反应大幅减少；从9.2%降至2.3%。
结论
使用安全简便采集的尿液样本进行ELISA检测，将成为肺吸虫病大规模筛查的有用工具。
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