从肺炎链球菌6B型荚膜多糖的碱性和酸性水解产物中分离含磷酸寡糖并进行结构研究。

Isolation and structural studies of phosphate-containing oligosaccharides from alkaline and acid hydrolysates of Streptococcus pneumoniae type 6B capsular polysaccharide.

作者信息

van Dam J E, Breg J, Komen R, Kamerling J P, Vliegenthart J F

机构信息

Department of Bio-Organic Chemistry, Utrecht University, The Netherlands.

出版信息

Carbohydr Res. 1989 Apr 15;187(2):267-86. doi: 10.1016/0008-6215(89)80008-4.

DOI:10.1016/0008-6215(89)80008-4

PMID:2743350

Abstract

The capsular polysaccharide of Streptococcus pneumoniae serotype 6B [----2)-alpha-D-Galp-(1----3)-alpha-D-Glcp-(1----3)-alpha-L-Rhap-( 1----4)- D-RibOH-(5-P----]n was depolymerised under alkaline (NaOH) and acidic (HF) conditions. The former treatment yielded, as the major component, alpha-2-P-Galp-(1----3)-alpha-Glcp-(1----3)-alpha-Rhap-(1----4)-5- P-RibOH. The latter treatment at -16 degrees gave alpha-Galp-(1----3)-alpha-Glcp-(1----3)-alpha-Rhap-(1----4)-Rib OH-(5-P----2)- alpha-Galp-(1----3)-alpha-Glcp-(1----3)-alpha-Rhap-(1----4)-Rib OH and at 4 degrees gave alpha-Galp-(1----3)-alpha-Glcp-(1----3)-alpha-Rhap-(1----4)-Rib OH. These oligosaccharides were characterised by sugar analysis, f.a.b.-m.s., and 1H- and 13C-n.m.r. spectroscopy.

摘要

肺炎链球菌6B血清型的荚膜多糖[----2)-α-D-半乳糖-(1----3)-α-D-葡萄糖-(1----3)-α-L-鼠李糖-(1----4)-D-核糖醇-(5-P----]n在碱性（NaOH）和酸性（HF）条件下解聚。前一种处理产生的主要成分是α-2-P-半乳糖-(1----3)-α-葡萄糖-(1----3)-α-鼠李糖-(1----4)-5-P-核糖醇。后一种处理在-16℃时产生α-半乳糖-(1----3)-α-葡萄糖-(1----3)-α-鼠李糖-(1----4)-核糖醇-(5-P----2)-α-半乳糖-(1----3)-α-葡萄糖-(1----3)-α-鼠李糖-(1----4)-核糖醇，在4℃时产生α-半乳糖-(1----3)-α-葡萄糖-(1----3)-α-鼠李糖-(1----4)-核糖醇。这些寡糖通过糖分析、快原子轰击质谱以及1H-和13C-核磁共振光谱进行表征。