van Dam J E, Breg J, Komen R, Kamerling J P, Vliegenthart J F
Department of Bio-Organic Chemistry, Utrecht University, The Netherlands.
Carbohydr Res. 1989 Apr 15;187(2):267-86. doi: 10.1016/0008-6215(89)80008-4.
The capsular polysaccharide of Streptococcus pneumoniae serotype 6B [----2)-alpha-D-Galp-(1----3)-alpha-D-Glcp-(1----3)-alpha-L-Rhap-( 1----4)- D-RibOH-(5-P----]n was depolymerised under alkaline (NaOH) and acidic (HF) conditions. The former treatment yielded, as the major component, alpha-2-P-Galp-(1----3)-alpha-Glcp-(1----3)-alpha-Rhap-(1----4)-5- P-RibOH. The latter treatment at -16 degrees gave alpha-Galp-(1----3)-alpha-Glcp-(1----3)-alpha-Rhap-(1----4)-Rib OH-(5-P----2)- alpha-Galp-(1----3)-alpha-Glcp-(1----3)-alpha-Rhap-(1----4)-Rib OH and at 4 degrees gave alpha-Galp-(1----3)-alpha-Glcp-(1----3)-alpha-Rhap-(1----4)-Rib OH. These oligosaccharides were characterised by sugar analysis, f.a.b.-m.s., and 1H- and 13C-n.m.r. spectroscopy.
肺炎链球菌6B血清型的荚膜多糖[----2)-α-D-半乳糖-(1----3)-α-D-葡萄糖-(1----3)-α-L-鼠李糖-(1----4)-D-核糖醇-(5-P----]n在碱性（NaOH）和酸性（HF）条件下解聚。前一种处理产生的主要成分是α-2-P-半乳糖-(1----3)-α-葡萄糖-(1----3)-α-鼠李糖-(1----4)-5-P-核糖醇。后一种处理在-16℃时产生α-半乳糖-(1----3)-α-葡萄糖-(1----3)-α-鼠李糖-(1----4)-核糖醇-(5-P----2)-α-半乳糖-(1----3)-α-葡萄糖-(1----3)-α-鼠李糖-(1----4)-核糖醇，在4℃时产生α-半乳糖-(1----3)-α-葡萄糖-(1----3)-α-鼠李糖-(1----4)-核糖醇。这些寡糖通过糖分析、快原子轰击质谱以及1H-和13C-核磁共振光谱进行表征。
